Rapid and sensitive analytical method for the determination of insulin in liposomes by reversed-phase HPLC

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorDentistry and Nursing-
Autor(es): dc.creatorde Souza Von Zuben, Eliete [UNESP]-
Autor(es): dc.creatorEloy, Josimar Oliveira-
Autor(es): dc.creatorSousa Araujo, Victor Hugo [UNESP]-
Autor(es): dc.creatorDaflon Gremião, Maria Palmira [UNESP]-
Autor(es): dc.creatorChorilli, Marlus [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:49:50Z-
Data de disponibilização: dc.date.available2022-02-22T00:49:50Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.17344/acsi.2020.6177-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207308-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207308-
Descrição: dc.descriptionInsulin is an important anabolic hormone that regulates the metabolism of carbohydrates, lipids and proteins. In this study, a reverse-phase liquid chromatography (RP-LC) method was successfully validated and tested for the encapsulation efficiency assay of insulin and in vitro release studies. HPLC analyses were carried out using a RP C18- Luna® Phenomenex (4.6 × 250 mm, 5 μm particle size) column maintained at room temperature, using a mobile phase constituted by a mixture of acetonitrile and 0.1% TFA aqueous solution (60:40, v/v), in an isocratic mode with a flow rate of 1.0 mL/ min, with ultraviolet detection at 214 nm and 20 μL of injection volume. Method validation was performed according recognized guidelines for system suitability, specificity, linearity, precision, accuracy, LOD, LOQ and robustness. The method was shown to be linear in the range of 0.5–100 µg/mL (r2 = 0.9993) selective, precise, robust, accurate with LOD and LOQ values were 0.097 µg/mL and 0.294 µg/mL, respectively. The developed method proved to be adequate to analyze the encapsulation efficiency and the profile of insulin release from liposomes.-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Drugs and Medicines, Rodovia Araraquara-Jaú, km1-
Descrição: dc.descriptionFederal University of Ceará School of Pharmacy Dentistry and Nursing Department of Pharmacy-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Drugs and Medicines, Rodovia Araraquara-Jaú, km1-
Formato: dc.format1273-1280-
Idioma: dc.languageen-
Relação: dc.relationActa Chimica Slovenica-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEncapsulation Efficiency-
Palavras-chave: dc.subjectIn Vitro Release-
Palavras-chave: dc.subjectInsulin-
Palavras-chave: dc.subjectLiposome-
Palavras-chave: dc.subjectLiquid Chromatography-
Palavras-chave: dc.subjectValidation Method-
Título: dc.titleRapid and sensitive analytical method for the determination of insulin in liposomes by reversed-phase HPLC-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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