2-acetylphenothiazine protects L929 fibroblasts against UVB-induced oxidative damage

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorState University of Maringá-
Autor(es): dc.contributorUniversidade Estadual de Maringá (UEM)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorda Silva, Bruna Terra Alves-
Autor(es): dc.creatorPeloi, Karen Elaine-
Autor(es): dc.creatorXimenes, Valdecir Farias [UNESP]-
Autor(es): dc.creatorNakamura, Celso Vataru-
Autor(es): dc.creatorde Oliveira Silva Lautenschlager, Sueli-
Data de aceite: dc.date.accessioned2022-02-22T00:49:36Z-
Data de disponibilização: dc.date.available2022-02-22T00:49:36Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jphotobiol.2021.112130-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207232-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207232-
Descrição: dc.descriptionUltraviolet B (UVB) light corresponds to 5% of ultraviolet radiation. It is more genotoxic and mutagenic than UVA and causes direct and indirect cellular damage through the generation of reactive oxygen species (ROS). Even after radiation, ROS generation may continue through activation of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) enzyme. Long-term exposure can progress to premature skin aging and photocarcinogenesis. To prevent damage that is caused by UVB radiation, several studies have focused on the topical administration of compounds that have antioxidant properties. 2-Acetylphenothiazine (ML171) is a potent and selective inhibitor of NOX1. The present study investigated the antioxidant potential and photoprotective ability of ML171 in UVB-irradiated L929 fibroblasts. ML171 had considerable antioxidant activity in both the DPPH• and xanthine/luminol/xanthine oxidase assays. ML171 did not induce cytotoxicity in L929 fibroblasts and increased the viability of UVB-irradiated cells. ML171 also inhibited ROS production, the enzymatic activity of NOX, depolarization of the mitochondrial membrane, and DNA damage. Additionally, ML171 protected cell membrane integrity and induced fibroblast migration. These results suggest that the incorporation of ML171 in topical administration systems may be a promising strategy to mitigate UVB-induced oxidative damage in L929 fibroblasts.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionPost-Graduate Program in Pharmaceutical Sciences State University of Maringá, Maringá-
Descrição: dc.descriptionDepartment of Basic Health Sciences Maringa State University (UEM), Maringá-
Descrição: dc.descriptionDepartment of Chemistry Faculty of Sciences São Paulo State University (UNESP), Bauru-
Descrição: dc.descriptionDepartment of Chemistry Faculty of Sciences São Paulo State University (UNESP), Bauru-
Descrição: dc.descriptionFAPESP: 2014/50926-0-
Descrição: dc.descriptionFAPESP: 2019/18445-5-
Idioma: dc.languageen-
Relação: dc.relationJournal of Photochemistry and Photobiology B: Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject2-acetylphenothiazine-
Palavras-chave: dc.subjectAntioxidant-
Palavras-chave: dc.subjectOxidative stress-
Palavras-chave: dc.subjectPhotoprotection-
Título: dc.title2-acetylphenothiazine protects L929 fibroblasts against UVB-induced oxidative damage-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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