A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of Rio de Janeiro-
Autor(es): dc.contributorScientific Board-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorTeixeira-Cruz, Jhonatha Mota-
Autor(es): dc.creatorStrauch, Marcelo Abrahão-
Autor(es): dc.creatorMonteiro-Machado, Marcos-
Autor(es): dc.creatorTavares-Henriques, Matheus Silva-
Autor(es): dc.creatorde Moraes, João Alfredo-
Autor(es): dc.creatorRibeiro da Cunha, Luís Eduardo-
Autor(es): dc.creatorFerreira, Rui Seabra [UNESP]-
Autor(es): dc.creatorBarraviera, Benedito [UNESP]-
Autor(es): dc.creatorQuintas, Luis Eduardo M-
Autor(es): dc.creatorMelo, Paulo A.-
Data de aceite: dc.date.accessioned2022-02-22T00:49:33Z-
Data de disponibilização: dc.date.available2022-02-22T00:49:33Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-01-04-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/toxins13010030-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207217-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207217-
Descrição: dc.descriptionMassive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local and systemic effects after envenomation, and there is no specific treatment for this potentially lethal event. This study evaluated the ability of a new Apilic antivenom, which is composed of F(ab')2 fraction of specific immunoglobulins in heterologous and hyperimmune equine serum, to neutralize A. mellifera venom and melittin, in vitro and in vivo, in mice. Animal experiments were performed in according with local ethics committee license (UFRJ protocol no. DFBCICB072-04/16). Venom dose-dependent lethality was diminished with 0.25-0.5 μL of intravenous Apilic antivenom/μg honeybee venom. In vivo injection of 0.1-1 μg/g bee venom induced myotoxicity, hemoconcentration, paw edema, and increase of vascular permeability which were antagonized by Apilic antivenom. Cytotoxicity, assessed in renal LLC-PK1 cells and challenged with 10 μg/mL honeybee venom or melittin, was neutralized by preincubation with Apilic antivenom, as well the hemolytic activity. Apilic antivenom inhibited phospholipase and hyaluronidase enzymatic activities. In flow cytometry experiments, Apilic antivenom neutralized reduction of cell viability due to necrosis by honeybee venom or melittin. These results showed that this antivenom is effective inhibitor of honeybee venom actions. Thus, this next generation of Apilic antivenom emerges as a new promising immunobiological product for the treatment of massive, Africanized honeybee attacks.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)-
Descrição: dc.descriptionGraduate Program in Pharmacology and Medicinal Chemistry Institute of Biomedical Sciences Federal University of Rio de Janeiro-
Descrição: dc.descriptionScientific Board-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)-
Descrição: dc.descriptionCAPES: 23038.006285/2011-21-
Descrição: dc.descriptionCNPq: 401170/2013-6-
Descrição: dc.descriptionCNPq: 409436/2016-0-
Descrição: dc.descriptionFAPERJ: e-26/200.006/2018-
Descrição: dc.descriptionFAPERJ: E_02/2017-
Idioma: dc.languageen-
Relação: dc.relationToxins-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectApilic antivenom-
Palavras-chave: dc.subjectApis mellifera-
Palavras-chave: dc.subjectenvenomation-
Palavras-chave: dc.subjecthoneybee venom-
Palavras-chave: dc.subjectmelittin-
Palavras-chave: dc.subjectpreclinical study-
Título: dc.titleA Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.