Immunocytochemistry Analysis of HepG2 Cell 3D Culture Encapsulated as Spheroids in Alginate Beads

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal Fluminense (UFF)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorBotucatu-
Autor(es): dc.creatorMiranda, Raul Ghiraldelli-
Autor(es): dc.creatorFerraz, Elisa Raquel Anastácio-
Autor(es): dc.creatorPereira, Lilian Cristina [UNESP]-
Autor(es): dc.creatorDorta, Daniel Junqueira [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:49:24Z-
Data de disponibilização: dc.date.available2022-02-22T00:49:24Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/978-1-0716-1091-6_14-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207167-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207167-
Descrição: dc.description3D Cell culture is an alternative to animal use in many drug development and toxicity studies. The 3D cell culture can mimic and reproduce the original tissue microenvironment, morphology, and mechanical and physiological characteristics, to provide a more realistic and reliable response as compared to two-dimensional cultures. 3D cell culture encapsulated in alginate beads is a very simple and relatively inexpensive tool that is easy to handle and to maintain. The alginate beads function as a scaffold that imprisons cells and allows 3D cell growth, to generate spheroids that can have greater genic expression and cell–cell communication as a nano or microtissue. The HepG2 cell line is a human hepatocellular carcinoma cell derivative. HepG2 cells preserve several of the characteristics of hepatocytes and are therefore often used in toxicity studies. Here, we describe HepG2 cell encapsulation in alginate beads and analyze the resulting spheroids formed within the alginate beads by immunocytochemistry, by staining a certain structure with a specific antibody coupled with a fluorophore. This method preserves the beads and enables cell analysis by confocal microscopy.-
Descrição: dc.descriptionDepartamento de Química Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo-
Descrição: dc.descriptionDepartment of Clinical Toxicological and Bromatological Analysis Faculty of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo-
Descrição: dc.descriptionFaculdade de Farmácia Universidade Federal Fluminense-
Descrição: dc.descriptionDepartment of Bioprocesses and Biotechnology Faculty of Agronomic Sciences of Botucatu São Paulo State University-
Descrição: dc.descriptionCenter for Evaluation of Environmental Impact on Human Health (TOXICAM) Botucatu-
Descrição: dc.descriptionFFCLRP-USP Department of Chemistry Faculty of Philosophy Sciences and Letters of Ribeirão Preto University of São Paulo Ribeirão Preto-
Descrição: dc.descriptionInstituto Nacional de Tecnologias Alternativas de Detecção Avaliação Toxicologicae Remoção de Micropututantes e Radioativos (INCT-DATREM) Unesp Instituto de Química-
Descrição: dc.descriptionDepartment of Bioprocesses and Biotechnology Faculty of Agronomic Sciences of Botucatu São Paulo State University-
Descrição: dc.descriptionInstituto Nacional de Tecnologias Alternativas de Detecção Avaliação Toxicologicae Remoção de Micropututantes e Radioativos (INCT-DATREM) Unesp Instituto de Química-
Formato: dc.format197-206-
Idioma: dc.languageen-
Relação: dc.relationMethods in Molecular Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject3D culture-
Palavras-chave: dc.subjectAlginate beads-
Palavras-chave: dc.subjectConfocal microscopy-
Palavras-chave: dc.subjectEncapsulation-
Palavras-chave: dc.subjectHepG2-
Palavras-chave: dc.subjectImmunocytochemistry-
Título: dc.titleImmunocytochemistry Analysis of HepG2 Cell 3D Culture Encapsulated as Spheroids in Alginate Beads-
Aparece nas coleções:Repositório Institucional - Unesp

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