Fractal dimension analysis: A new tool for analyzing colony-forming units

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Autor(es): dc.contributorUniversidade do Oeste Paulista (UNOESTE)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorNai, Gisele Alborghetti-
Autor(es): dc.creatorMartelli, Cesar Alberto TALAvera-
Autor(es): dc.creatorMedina, Denis Aloísio Lopes-
Autor(es): dc.creatorOliveira, Mayla Silva Cayres de-
Autor(es): dc.creatorCaldeira, Isadora Delfino-
Autor(es): dc.creatorHenriques, Bruno Carvalho-
Autor(es): dc.creatorPortelinha, Maria Júlia Schadeck-
Autor(es): dc.creatorEller, Lizziane Kretli Winkelstroter-
Autor(es): dc.creatorMarques, Mariângela Esther Alencar [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:49:20Z-
Data de disponibilização: dc.date.available2022-02-22T00:49:20Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.mex.2021.101228-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207144-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207144-
Descrição: dc.descriptionThe gold standard for quantifying bacteria both in routine diagnostics and in research is plating followed by count of colony-forming units (CFU). But, manual CFU counting on plates is time-consuming and subjective. We evaluated fractal dimension as a new methodology for evaluating CFU. Twenty fragments of expanded polytetrafluoroethylene (ePTFE) synthetic vascular prosthesis and 20 silicone prostheses were embedded in bacterial suspensions and incubated. The prostheses were then sown in solid culture medium and incubated for 48 h. Petri dishes were photographed and analyzed by fractal dimension. There was correlation between the number of CFU in manual counting and the fractal dimension analysis (p = 0.0001). We demonstrated that fractal dimension is a useful method for microbiological analyses in researches. It makes CFU analysis easier and faster and can be used regardless of the culture medium. • Petri dishes with different bacterial colonies were photographed with a digital camera under natural light. • The images were binarized and analyzed with ImageJⓇ's “fractal dimension” tool. • Fractal dimension analysis showed to be a good tool for evaluating the amount of colony-forming unit.-
Descrição: dc.descriptionDepartment of Pathology Universidade do Oeste Paulista (UNOESTE), Rua José Bongiovani, 700-
Descrição: dc.descriptionDepartment of Surgery Universidade do Oeste Paulista (UNOESTE), Rua José Bongiovani, 700-
Descrição: dc.descriptionLaboratory of Clinical Analysis Universidade do Oeste Paulista (UNOESTE), Rua José Bongiovani, 700-
Descrição: dc.descriptionMedical College Universidade do Oeste Paulista (UNOESTE), Rua José Bongiovani, 700-
Descrição: dc.descriptionDepartment of Microbiology Graduate Program in Health Sciences Universidade do Oeste Paulista (UNOESTE), Rua José Bongiovani, 700-
Descrição: dc.descriptionDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP), Rubião Junior, s/n, 18618-970-
Descrição: dc.descriptionDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP), Rubião Junior, s/n, 18618-970-
Idioma: dc.languageen-
Relação: dc.relationMethodsX-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectColony-forming units counting (CFU)-
Palavras-chave: dc.subjectComputer-assisted-
Palavras-chave: dc.subjectImage processing-
Palavras-chave: dc.subjectMicrobial colony count-
Palavras-chave: dc.subjectProstheses and implants-
Título: dc.titleFractal dimension analysis: A new tool for analyzing colony-forming units-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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