In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of Alfenas (UNIFAL)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorMazzoni, Talita Sarah [UNESP]-
Autor(es): dc.creatorQuagio-Grassiotto, Irani [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:49:13Z-
Data de disponibilização: dc.date.available2022-02-22T00:49:13Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/jemt.23695-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207099-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207099-
Descrição: dc.descriptionParaffin has been the most traditional embedding medium used in histological techniques, however it can cause several technical artifacts. On the other hand, the use of plastic resins gives a more consistent and precise support to the tissues, allowing a better perfection of the histological sections. Thus, in an attempt to retrieve samples previously embedded in paraffin, we have described this new protocol which allows the material to be deparaffinized and re-embedded into historesin (glycol methacrylate). Paraffin embedded biological materials (en bloc) were deparaffinized and re-embedded with historesin. The histological sections of the samples (in paraffin and historesin) were analyzed under light microscope and the quality of the material was compared. As expected, samples embedded in historesin showed a better quality in their morphology, even if they were previously embedded in paraffin. Therefore, this new technique proposed here allows the recovery of old materials, through reprocessing to historesin, ensuring that materials already collected that cannot be obtained again are analyzed with greater clarity and great detail.-
Descrição: dc.descriptionDepartment of Cell and Developmental Biology Institute of Biomedical Sciences Federal University of Alfenas (UNIFAL)-
Descrição: dc.descriptionDepartment of Morphology Institute of Biosciences of Botucatu São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Morphology Institute of Biosciences of Botucatu São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationMicroscopy Research and Technique-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcytochemistry-
Palavras-chave: dc.subjectembedding medium-
Palavras-chave: dc.subjectglycol methacrylate-
Palavras-chave: dc.subjecthistology-
Palavras-chave: dc.subjectparaffin-
Título: dc.titleIn totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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