Combined Biomaterials: Amniotic Membrane and Adipose Tissue to Restore Injured Bone as Promoter of Calcification in Bone Regeneration: Preclinical Model

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Autor(es): dc.contributorChild and Adolescent Health Research & Pequeno Príncipe Faculties-
Autor(es): dc.contributorPositivo University-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorThe Institute of Biological and Health Sciences of the Pontifical Catholic University-
Autor(es): dc.contributorNorthwestern University-
Autor(es): dc.contributorFederal University of Paraná-
Autor(es): dc.creatorDziedzic, Dilcele Silva Moreira-
Autor(es): dc.creatorFrancisco, Júlio César-
Autor(es): dc.creatorMogharbel, Bassam Felipe-
Autor(es): dc.creatorIrioda, Ana Carolina-
Autor(es): dc.creatorStricker, Priscila Elias Ferreira-
Autor(es): dc.creatorFloriano, Juliana [UNESP]-
Autor(es): dc.creatorde Noronha, Lúcia-
Autor(es): dc.creatorAbdelwahid, Eltyeb-
Autor(es): dc.creatorFranco, Célia Regina Cavichiolo-
Autor(es): dc.creatorde Carvalho, Katherine Athayde Teixeira-
Data de aceite: dc.date.accessioned2022-02-22T00:49:11Z-
Data de disponibilização: dc.date.available2022-02-22T00:49:11Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00223-020-00793-1-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207090-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207090-
Descrição: dc.descriptionDiscarded tissues, like human amniotic membranes and adipose tissue, were investigated for the application of Decellularized Human Amniotic Membrane (DAM) as a viable scaffold for transplantation of Adipose-derived stromal cells (ASCs) in bone regeneration of non-healing calvarial defects in rats. Amniotic membrane was decellularized to provide a scaffold for male Wistar rats ASCs expansion and transplantation. ASCs osteoinduction in vitro promoted the deposition of a mineralized bone-like matrix by ASCs, as calcified globular accretions associated with the cells on the DAM surface and inside the collagenous matrix. Non-healing calvarial defects on male Wistar rats were randomly divided in control without treatment, treatment with four layers of DAM, or four layers of DAM associated with ASCs. After 12 weeks, tissue blocks were examined by micro-computed tomography and histology. DAM promoted osteoconduction by increasing the collagenous matrix on both DAM treatments. DAM with ASCs stimulated bone deposition, demonstrated by a higher percentage of bone volume and trabecular bone number, compared to control. Besides the osteogenic capacity in vitro, ASCs stimulated the healing of calvarial defects with significant DAM graft incorporation concomitant with higher host bone deposition. The enhanced in vivo bone regeneration by undifferentiated ASCs loaded onto DAM confirmed the potential of an easily collected autologous cell source associated with a broadly available collagenous matrix in tissue engineering.-
Descrição: dc.descriptionCell Therapy and Biotechnology in Regenerative Medicine Department The Pelé Pequeno Príncipe Institute Child and Adolescent Health Research & Pequeno Príncipe Faculties, Ave. Silva Jardim, no. 1632, Box 80240-020-
Descrição: dc.descriptionPositivo University, St.Professor Pedro Viriato Parigot de Souza, Box 80710-570-
Descrição: dc.descriptionPhysics Department São Paulo State University (UNESP), Ave. Eng. Luís Edmundo Carrijo Coube, 2085 - Núcleo Res. Pres. Geisel, Box 17033-360-
Descrição: dc.descriptionPathology Department The Institute of Biological and Health Sciences of the Pontifical Catholic University, Ave. Imaculada Conceição, 1155, Box 80215-901-
Descrição: dc.descriptionFeinberg School of Medicine Feinberg Cardiovascular Research Institute Northwestern University, 303 E. Chicago Ave., Tarry 14–725-
Descrição: dc.descriptionCell Biology Department Federal University of Paraná, Ave. Coronel Francisco Heráclito dos Santos 210, Box 81531-970-
Descrição: dc.descriptionPhysics Department São Paulo State University (UNESP), Ave. Eng. Luís Edmundo Carrijo Coube, 2085 - Núcleo Res. Pres. Geisel, Box 17033-360-
Formato: dc.format667-679-
Idioma: dc.languageen-
Relação: dc.relationCalcified Tissue International-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAdipose stem cells-
Palavras-chave: dc.subjectBone tissue engineering-
Palavras-chave: dc.subjectHuman amniotic membrane-
Palavras-chave: dc.subjectStem cell transplantation-
Título: dc.titleCombined Biomaterials: Amniotic Membrane and Adipose Tissue to Restore Injured Bone as Promoter of Calcification in Bone Regeneration: Preclinical Model-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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