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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Child and Adolescent Health Research & Pequeno Príncipe Faculties | - |
Autor(es): dc.contributor | Positivo University | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | The Institute of Biological and Health Sciences of the Pontifical Catholic University | - |
Autor(es): dc.contributor | Northwestern University | - |
Autor(es): dc.contributor | Federal University of Paraná | - |
Autor(es): dc.creator | Dziedzic, Dilcele Silva Moreira | - |
Autor(es): dc.creator | Francisco, Júlio César | - |
Autor(es): dc.creator | Mogharbel, Bassam Felipe | - |
Autor(es): dc.creator | Irioda, Ana Carolina | - |
Autor(es): dc.creator | Stricker, Priscila Elias Ferreira | - |
Autor(es): dc.creator | Floriano, Juliana [UNESP] | - |
Autor(es): dc.creator | de Noronha, Lúcia | - |
Autor(es): dc.creator | Abdelwahid, Eltyeb | - |
Autor(es): dc.creator | Franco, Célia Regina Cavichiolo | - |
Autor(es): dc.creator | de Carvalho, Katherine Athayde Teixeira | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:49:11Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:49:11Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-05-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1007/s00223-020-00793-1 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/207090 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/207090 | - |
Descrição: dc.description | Discarded tissues, like human amniotic membranes and adipose tissue, were investigated for the application of Decellularized Human Amniotic Membrane (DAM) as a viable scaffold for transplantation of Adipose-derived stromal cells (ASCs) in bone regeneration of non-healing calvarial defects in rats. Amniotic membrane was decellularized to provide a scaffold for male Wistar rats ASCs expansion and transplantation. ASCs osteoinduction in vitro promoted the deposition of a mineralized bone-like matrix by ASCs, as calcified globular accretions associated with the cells on the DAM surface and inside the collagenous matrix. Non-healing calvarial defects on male Wistar rats were randomly divided in control without treatment, treatment with four layers of DAM, or four layers of DAM associated with ASCs. After 12 weeks, tissue blocks were examined by micro-computed tomography and histology. DAM promoted osteoconduction by increasing the collagenous matrix on both DAM treatments. DAM with ASCs stimulated bone deposition, demonstrated by a higher percentage of bone volume and trabecular bone number, compared to control. Besides the osteogenic capacity in vitro, ASCs stimulated the healing of calvarial defects with significant DAM graft incorporation concomitant with higher host bone deposition. The enhanced in vivo bone regeneration by undifferentiated ASCs loaded onto DAM confirmed the potential of an easily collected autologous cell source associated with a broadly available collagenous matrix in tissue engineering. | - |
Descrição: dc.description | Cell Therapy and Biotechnology in Regenerative Medicine Department The Pelé Pequeno Príncipe Institute Child and Adolescent Health Research & Pequeno Príncipe Faculties, Ave. Silva Jardim, no. 1632, Box 80240-020 | - |
Descrição: dc.description | Positivo University, St.Professor Pedro Viriato Parigot de Souza, Box 80710-570 | - |
Descrição: dc.description | Physics Department São Paulo State University (UNESP), Ave. Eng. Luís Edmundo Carrijo Coube, 2085 - Núcleo Res. Pres. Geisel, Box 17033-360 | - |
Descrição: dc.description | Pathology Department The Institute of Biological and Health Sciences of the Pontifical Catholic University, Ave. Imaculada Conceição, 1155, Box 80215-901 | - |
Descrição: dc.description | Feinberg School of Medicine Feinberg Cardiovascular Research Institute Northwestern University, 303 E. Chicago Ave., Tarry 14–725 | - |
Descrição: dc.description | Cell Biology Department Federal University of Paraná, Ave. Coronel Francisco Heráclito dos Santos 210, Box 81531-970 | - |
Descrição: dc.description | Physics Department São Paulo State University (UNESP), Ave. Eng. Luís Edmundo Carrijo Coube, 2085 - Núcleo Res. Pres. Geisel, Box 17033-360 | - |
Formato: dc.format | 667-679 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Calcified Tissue International | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Adipose stem cells | - |
Palavras-chave: dc.subject | Bone tissue engineering | - |
Palavras-chave: dc.subject | Human amniotic membrane | - |
Palavras-chave: dc.subject | Stem cell transplantation | - |
Título: dc.title | Combined Biomaterials: Amniotic Membrane and Adipose Tissue to Restore Injured Bone as Promoter of Calcification in Bone Regeneration: Preclinical Model | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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