Improved in vitro and in vivo anti-candida albicans activity of cymbopogon nardus essential oil by its incorporation into a microemulsion system

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorInstitute of Biological Sciences-
Autor(es): dc.contributorScience and Technology (IFSP)-
Autor(es): dc.contributorFaculty of Medicine of São José do Rio Preto (FAMERP)-
Autor(es): dc.creatorde Toledo, Luciani Gaspar [UNESP]-
Autor(es): dc.creatorRamos, Matheus Aparecido Dos Santos [UNESP]-
Autor(es): dc.creatorda Silva, Patrícia Bento-
Autor(es): dc.creatorRodero, Camila Fernanda [UNESP]-
Autor(es): dc.creatorde Sá Gomes, Veridiana [UNESP]-
Autor(es): dc.creatorda Silva, Anderson Noronha [UNESP]-
Autor(es): dc.creatorPavan, Fernando Rogério [UNESP]-
Autor(es): dc.creatorda Silva, Isabel Cristiane [UNESP]-
Autor(es): dc.creatorOda, Fernando Bombarda [UNESP]-
Autor(es): dc.creatorFlumignan, Danilo Luis-
Autor(es): dc.creatorDos Santos, André Gonzaga [UNESP]-
Autor(es): dc.creatorChorilli, Marlus [UNESP]-
Autor(es): dc.creatorde Almeida, Margarete Teresa Gottardo-
Autor(es): dc.creatorBauab, Taís Maria [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:49:07Z-
Data de disponibilização: dc.date.available2022-02-22T00:49:07Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.2147/IJN.S275258-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207075-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207075-
Descrição: dc.descriptionPurpose: Vulvovaginal candidiasis (VVC) is an opportunistic fungal infection that adversely affects a woman’s health, due to unpleasant symptoms, therapeutic challenges, and the emergence of resistant strains. The association of natural products and nanotechnol-ogy is important to improve the antifungal potential of medicinal plants. We aimed to evaluate the in vitro and in vivo anti-Candida albicans activity of unloaded (EO) and loaded (ME+EO) essential oil of Cymbopogon nardus in the microemulsion (ME). Methods: The chemical analysis of the EO was performed by gas chromatography-mass spectrometry. The ME and ME+EO were characterized by scattering, zeta potential, polar-ized light microscopy, rheological assays, mucoadhesiveness and transmission electronic microscopy. The in vitro antifungal activity of the EO and ME+EO were evaluated by microdilution technique. The toxicity of EO and ME+EO was analyzed on human cell line HaCat and using alternative model assay with Artemia salina. The experimental in vivo VVC was performed in female mice (C57BL/6). Results: The main compounds of the EO were found to be citronellal, geranial, geraniol, citronellol, and neral. The formulations exhibited suitable size, homogeneity, negative charge, isotropic behavior, highly organized structure, and pseudoplastic behavior, for vaginal applica-tion. TEM photomicrographs showed possible EO droplets inside the spherical structures. The EO, when loaded into the ME, exhibited an improvement in its antifungal action against C. albicans. The EO was not toxic against brine shrimp nauplii. An in vivo VVC assay showed that the use of the ME significantly improved the action of the EO, since only the ME+EO promoted the eradication of the fungal vaginal infection on the third day of treatment. Conclusion: The EO and ME+EO are promising alternatives for the control of fungal infections caused by C. albicans, once the use of nanotechnology significantly improved the antifungal action of the EO, especially in an in vivo model of VVC.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences Department of Biological Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Genetics and Morphology University of Brasília (UnB) Institute of Biological Sciences, Distrito Federal-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences Department of Drug and Medicines São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences Department of Natural Active Principles and Toxicology São Paulo State University (UNESP)-
Descrição: dc.descriptionSão Paulo Federal Institute of Education Science and Technology (IFSP)-
Descrição: dc.descriptionFaculty of Medicine of São José do Rio Preto (FAMERP) Department of Infectious Diseases-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences Department of Biological Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences Department of Drug and Medicines São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences Department of Natural Active Principles and Toxicology São Paulo State University (UNESP)-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2014/50926-0-
Descrição: dc.descriptionFAPESP: 2016/08559-5-
Descrição: dc.descriptionFAPESP: 2017-21356-9-
Descrição: dc.descriptionFAPESP: 2020/13057-4-
Formato: dc.format10481-10497-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Nanomedicine-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCitronella-
Palavras-chave: dc.subjectNanostructured lipid system-
Palavras-chave: dc.subjectTherapeutic treatment-
Palavras-chave: dc.subjectVulvovaginal candidiasis-
Título: dc.titleImproved in vitro and in vivo anti-candida albicans activity of cymbopogon nardus essential oil by its incorporation into a microemulsion system-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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