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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Laboratory of Microbiology Applied to Aquatic Organisms | - |
Autor(es): dc.contributor | Laboratory of Parasitology and Pathology of Aquatic Organisms | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Aquaculture and Fisheries Group | - |
Autor(es): dc.contributor | Laboratory of Microbiology and Advanced Immunology | - |
Autor(es): dc.creator | Gallani, Sílvia Umeda | - |
Autor(es): dc.creator | Valladão, Gustavo Moraes Ramos | - |
Autor(es): dc.creator | Alves, Lindomar de Oliveira [UNESP] | - |
Autor(es): dc.creator | Kotzent, Suzana [UNESP] | - |
Autor(es): dc.creator | Hashimoto, Diogo Teruo [UNESP] | - |
Autor(es): dc.creator | Wiegertjes, Geert | - |
Autor(es): dc.creator | Kirsten, Karina | - |
Autor(es): dc.creator | Kreutz, Luiz Carlos | - |
Autor(es): dc.creator | Pilarski, Fabiana [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:49:01Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:49:01Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2020-12-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1016/j.micpath.2020.104638 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/207038 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/207038 | - |
Descrição: dc.description | The production of tambaqui Colossoma macropomum has recently reached a milestone, being considered the main native species produced in South American continental waters. Despite the importance of this fish, its immunity is not well understood. In this study we established some patterns of innate immunity for the species via two experiments. Both studies evaluated the fish in the absence (intraperitoneal saline) or presence (intraperitoneal, 3 x 107 CFU/mL of Aeromonas hydrophila at 0.1 mL/10 g of living weight) of infection at 5 points over time-course of 14 days (0 h, 6 h, 24 h, 7 d, 14 d). In the first experiment, the partial gene sequences and gene expression of IL-1β, IRAK-1, C3, C4, lysozyme, IL-10, HSP70 and β-actin were determined in the main secondary lymphoid organs of fish: the spleen and head kidney. The second study was performed to analyse the alternative complement pathway ACH50 in serum to support the elucidation of C3 gene expression. Results of the gene expression assays showed a tendency towards up-regulation of immune genes in infected fish in early phases of infection (mostly around 6 h and 24 h) and in the chronic phase (7 d and 14 d), with the exception of HSP70 which showed a down-regulation in infected fish. Our results also suggested that lysozyme was evolved in both pro- and anti-inflammatory activities. For genes of the complement system, it was demonstrated that C4 regulation followed the tendency of pro-inflammatory genes. However, the C3 gene was, surprisingly, not expressed in most fish and this corroborated with the results of the complement system activity in serum that also did not show activity in most fish. The possible reasons for the regulation of gene expression and association with fish disease are addressed in this paper. | - |
Descrição: dc.description | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | - |
Descrição: dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
Descrição: dc.description | Nilton Lins University (UNL) Laboratory of Microbiology Applied to Aquatic Organisms | - |
Descrição: dc.description | Nilton Lins University (UNL) Laboratory of Parasitology and Pathology of Aquatic Organisms | - |
Descrição: dc.description | São Paulo State University (UNESP) Aquaculture Center of UNESP Laboratory of Microbiology and Parasitology of Aquatic Organisms | - |
Descrição: dc.description | São Paulo State University (UNESP) Aquaculture Center of UNESP Laboratory of Aquaculture Genetics and Conservation | - |
Descrição: dc.description | Wageningen University & Research (WUR) Aquaculture and Fisheries Group | - |
Descrição: dc.description | University of Passo Fundo (UPF) Faculty of Agronomy and Veterinary Medicine Laboratory of Microbiology and Advanced Immunology | - |
Descrição: dc.description | São Paulo State University (UNESP) Aquaculture Center of UNESP Laboratory of Microbiology and Parasitology of Aquatic Organisms | - |
Descrição: dc.description | São Paulo State University (UNESP) Aquaculture Center of UNESP Laboratory of Aquaculture Genetics and Conservation | - |
Descrição: dc.description | CAPES: 001 | - |
Descrição: dc.description | CNPq: 305007/2016-5 | - |
Descrição: dc.description | CNPq: 431713/2016-2 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Microbial Pathogenesis | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Aeromonas hydrophila | - |
Palavras-chave: dc.subject | Aquaculture | - |
Palavras-chave: dc.subject | Black pacu | - |
Palavras-chave: dc.subject | Complement system | - |
Palavras-chave: dc.subject | Interleukins | - |
Palavras-chave: dc.subject | Lysozyme | - |
Título: dc.title | Patterns of the innate immune response in tambaqui Colossoma macropomum: Modulation of gene expression in haemorrhagic septicaemia caused by Aeromonas hydrophila | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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