Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUNOESTE-University of West Paulista-
Autor(es): dc.creatorCataneli Pereira, Valéria [UNESP]-
Autor(es): dc.creatorPinheiro-Hubinger, Luiza [UNESP]-
Autor(es): dc.creatorde Oliveira, Adilson [UNESP]-
Autor(es): dc.creatorMoraes Riboli, Danilo Flávio [UNESP]-
Autor(es): dc.creatorBenini Martins, Katheryne [UNESP]-
Autor(es): dc.creatorCalixto Romero, Letícia [UNESP]-
Autor(es): dc.creatorRibeiro de Souza da Cunha, Maria de Lourdes [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:48:45Z-
Data de disponibilização: dc.date.available2022-02-22T00:48:45Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-02-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/molecules25235715-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206958-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206958-
Descrição: dc.descriptionThe ability of Staphylococcus epidermidis to produce virulence factors, such as biofilm, added to its increased resistance to antimicrobials can cause infections that are difficult to treat. Many staphylococcal virulence factors are under the control of the accessory gene regulator (agr). The objective of this study was to establish the agr locus and susceptibility of biofilm-producing S. epidermidis specimens to antimicrobial agents, through PCR reactions, reverse transcription polymerase chain reaction (RT-PCR), and the determination of minimum inhibitory concentration (MIC), and to analyze the clonal profile of 300 strains isolated from blood culture specimens from inpatients at a University Hospital in Brazil, over a 20-year period by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) techniques. The ica operon expression was shown in 83.6% strains, bhp gene in 11.5%, and aap gene in 32.8%. Oxacillin resistance was detected in 90.1%, while 4.9% showed tigecycline resistance, and intermediate resistance to quinupristin/dalfopristin was identified in 0.4%. Clonal profile determination showed 11 clusters, with the ST2 type determined as the major cluster. The S. epidermidis biofilm producer demonstrated a predominance of agr I locus, oxacillin resistance, and SCCmec III as well as the potential dissemination of pathogenic clones in hospital settings over long periods.-
Descrição: dc.descriptionSector of Microbiology and Immunology Department of Chemical and Biological Sciences Institute of Biosciences UNESP-University Estadual Paulista-
Descrição: dc.descriptionSector of Microbiology and Immunology UNOESTE-University of West Paulista-
Descrição: dc.descriptionSector of Microbiology and Immunology Department of Chemical and Biological Sciences Institute of Biosciences UNESP-University Estadual Paulista-
Idioma: dc.languageen-
Relação: dc.relationMolecules (Basel, Switzerland)-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectagr locus-
Palavras-chave: dc.subjectantimicrobial resistance-
Palavras-chave: dc.subjectbiofilm-
Palavras-chave: dc.subjectoxacillin resistance-
Palavras-chave: dc.subjectST2-
Título: dc.titleDetection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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