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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Royal Botanic Gardens | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | CSIC-Ajuntament de Barcelona) | - |
Autor(es): dc.creator | Kooij, Pepijn W. [UNESP] | - |
Autor(es): dc.creator | Pellicer, Jaume | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:48:41Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:48:41Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2019-12-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1093/GBE/EVAA217 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/206938 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/206938 | - |
Descrição: dc.description | Each day, as the amount of genomic data and bioinformatics resources grows, researchers are increasingly challenged with selecting the most appropriate approach to analyze their data. In addition, the opportunity to undertake comparative genomic analyses is growing rapidly. This is especially true for fungi due to their small genome sizes (i.e., mean 1C ¼ 44.2 Mb). Given these opportunities and aiming to gain novel insights into the evolution of mutualisms, we focus on comparing the quality of whole genome assemblies for fungus-growing ants cultivars (Hymenoptera: Formicidae: Attini) and a free-living relative. Our analyses reveal that currently available methodologies and pipelines for analyzing whole-genome sequence data need refining. By using different genome assemblers, we show that the genome assembly size depends on what software is used. This, in turn, impacts gene number predictions, with higher gene numbers correlating positively with genome assembly size. Furthermore, the majority of fungal genome size data currently available are based on estimates derived from whole-genome assemblies generated from short-read genome data, rather than from the more accurate technique of flow cytometry. Here, we estimated the haploid genome sizes of three ant fungal symbionts by flow cytometry using the fungus Pleurotus ostreatus (Jacq.) P. Kumm. (1871) as a calibration standard. We found that published genome sizes based on genome assemblies are 2.5- to 3-fold larger than our estimates based on flow cytometry. We, therefore, recommend that flow cytometry is used to precalibrate genome assembly pipelines, to avoid incorrect estimates of genome sizes and ensure robust assemblies. | - |
Descrição: dc.description | Department of Comparative Plant and Fungal Biology Royal Botanic Gardens | - |
Descrição: dc.description | Center for the Study of Social Insects São Paulo State University (UNESP) | - |
Descrição: dc.description | Institut Botanic de Barcelona (IBB CSIC-Ajuntament de Barcelona) | - |
Descrição: dc.description | Center for the Study of Social Insects São Paulo State University (UNESP) | - |
Formato: dc.format | 2384-2390 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Genome Biology and Evolution | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Evolution | - |
Palavras-chave: dc.subject | Fungi | - |
Palavras-chave: dc.subject | Fungus-growing ants | - |
Palavras-chave: dc.subject | Genome assembly | - |
Palavras-chave: dc.subject | Genome size | - |
Palavras-chave: dc.subject | Mutualism | - |
Título: dc.title | Genome size versus genome assemblies: Are the genomes truly expanded in polyploid fungal symbionts? | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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