Can Statistical Evaluation Tools for Chromatographic Method Development Assist in the Natural Products Workflow? A Case Study on Selected Species of the Plant Family Malpighiaceae

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorSan Diego-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorMax Planck Institute of Molecular Plant Physiology-
Autor(es): dc.contributorFeira de Santana State University (UEFS)-
Autor(es): dc.creatorMannochio-Russo, Helena [UNESP]-
Autor(es): dc.creatorBueno, Paula Carolina P.-
Autor(es): dc.creatorBauermeister, Anelize-
Autor(es): dc.creatorDe Almeida, Rafael Felipe-
Autor(es): dc.creatorDorrestein, Pieter C.-
Autor(es): dc.creatorCavalheiro, Alberto José [UNESP]-
Autor(es): dc.creatorBolzani, Vanderlan S. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:48:28Z-
Data de disponibilização: dc.date.available2022-02-22T00:48:28Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-11-24-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1021/acs.jnatprod.0c00495-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206868-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206868-
Descrição: dc.descriptionProper chromatographic methods may reduce the challenges inherent in analyzing natural product extracts, especially when utilizing hyphenated detection techniques involving mass spectrometry. As there are many variations one can introduce during chromatographic method development, this can become a daunting and time-consuming task. To reduce the number of runs and time needed, the use of instrumental automatization and commercial software to apply Quality by Design and statistical analysis automatically can be a valuable approach to investigate complex matrices. To evaluate this strategy in the natural products workflow, a mixture of nine species from the family Malpighiaceae was investigated. By this approach, the entire data collection and method development procedure (comprising screening, optimization, and robustness simulation) was accomplished in only 4 days, resulting in very low limits of detection and quantification. The analysis of the individual extracts also proved the efficiency of the use of a mixture of extracts for this workflow. Molecular networking and library searches were used to annotate a total of 61 compounds, including O-glycosylated flavonoids, C-glycosylated flavonoids, quinic/shikimic acid derivatives, sterols, and other phenols, which were efficiently separated by the method developed. These results support the potential of statistical tools for chromatographic method optimization as an efficient approach to reduce time and maximize resources, such as solvents, to get proper chromatographic conditions.-
Descrição: dc.descriptionNuBBE Department of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)-
Descrição: dc.descriptionCollaborative Mass Spectrometry Innovation Center Skaggs School of Pharmacy and Pharmaceutical Sciences University of California San Diego-
Descrição: dc.descriptionFaculty of Pharmaceutical Sciences of Ribeirão Preto Department of Physics and Chemistry University of São Paulo-
Descrição: dc.descriptionMax Planck Institute of Molecular Plant Physiology-
Descrição: dc.descriptionBiomedical Sciences Institute University of São Paulo-
Descrição: dc.descriptionDepartment of Biological Sciences Lamol Lab Feira de Santana State University (UEFS)-
Descrição: dc.descriptionNuBBE Department of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)-
Formato: dc.format3239-3249-
Idioma: dc.languageen-
Relação: dc.relationJournal of Natural Products-
???dc.source???: dc.sourceScopus-
Título: dc.titleCan Statistical Evaluation Tools for Chromatographic Method Development Assist in the Natural Products Workflow? A Case Study on Selected Species of the Plant Family Malpighiaceae-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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