In situ proteomic analysis of glioblastoma multiforme: a translational approach to improve prognostic/diagnostic routines

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorMenegasso, Anally Ribeiro da Silva [UNESP]-
Autor(es): dc.creatorPratavieira, Marcel [UNESP]-
Autor(es): dc.creatorSantos, Lucilene Delazari dos [UNESP]-
Autor(es): dc.creatorLima, Flávio de Oliveira [UNESP]-
Autor(es): dc.creatorMoraes, Marcelo Padovani T. [UNESP]-
Autor(es): dc.creatorZanini, Marco Antonio [UNESP]-
Autor(es): dc.creatorPalma, Mario Sergio [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:48:20Z-
Data de disponibilização: dc.date.available2022-02-22T00:48:20Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.30744/BRJAC.2179-3425.AR-41-2020-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206823-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206823-
Descrição: dc.descriptionIn order to evaluate the use of matrix-assisted laser desorption ionization (MALDI) Mass Spectral Imaging (MSI) to Glioblastoma (GBM) studies, some sections of formalin fixed paraffin embedded samples of GBM tumors were submitted to classical immunoassays, to define the profile of distribution of some classical and well recognized molecular markers of GBM grade IV (Ki-67, S100, Glial GFAP, CD31 and CD34), while other sections of the same samples were submitted to in-tissue proteomic analysis by MALDI MSI, and both results compared to each other. The overlapping of the MALDI spectra obtained for the tryptic peptides with the immunohistochemical reactions of each marker protein were used to build a distribution map of the marker proteins all over the GBM tissue section. The results revealed a high correlation between both methods, indicating that MALDI MSI has enough sensitivity to be compared to the immunohistochemical methods, as well is sufficiently reliable to be used in biomarkers identification.-
Descrição: dc.descriptionInstitute of Biosciences Department of Basic and Applied Biology Center of the Study of Social Insects Sao Paulo State University (UNESP)-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) Sao Paulo State University (UNESP)-
Descrição: dc.descriptionBotucatu Medical School (FMB) Sao Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Biosciences Department of Basic and Applied Biology Center of the Study of Social Insects Sao Paulo State University (UNESP)-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) Sao Paulo State University (UNESP)-
Descrição: dc.descriptionBotucatu Medical School (FMB) Sao Paulo State University (UNESP)-
Formato: dc.format64-83-
Idioma: dc.languageen-
Relação: dc.relationBrazilian Journal of Analytical Chemistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectGlioblastoma multiforme-
Palavras-chave: dc.subjectIn situ proteomics-
Palavras-chave: dc.subjectMALDI Imaging-
Palavras-chave: dc.subjectMALDI-TOF MS-
Palavras-chave: dc.subjectTumor biomarkers-
Título: dc.titleIn situ proteomic analysis of glioblastoma multiforme: a translational approach to improve prognostic/diagnostic routines-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.