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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Universidade de São Paulo (USP) | - |
Autor(es): dc.creator | de Oliveira Duque, C. C. [UNESP] | - |
Autor(es): dc.creator | Soares, D. G. | - |
Autor(es): dc.creator | Briso, A. L.F. [UNESP] | - |
Autor(es): dc.creator | Ortecho-Zuta, U. [UNESP] | - |
Autor(es): dc.creator | de Oliveira Ribeiro, R. A. [UNESP] | - |
Autor(es): dc.creator | Hebling, J. [UNESP] | - |
Autor(es): dc.creator | de Souza Costa, C. A. [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:48:20Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:48:20Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2020-10-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.2341/19-013-L | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/206822 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/206822 | - |
Descrição: dc.description | Objective: The aim of this study was to evaluate the influence of the presence of pigments in tooth structures on the trans-enamel and trans-dentin diffusion of hydrogen peroxide (H2O2) and its cytotoxicity after carrying out an in-office bleaching therapy. Methods and Materials: A bleaching gel with 35% H2O2 was applied for 45 minutes (three times for 15 minutes) on enamel and dentin discs (n=6), either previously submitted to the intrinsic pigmentation protocol with a concentrated solution of black tea, or not, defining the following groups: G1, unbleached untreated discs (control 1); G2, unbleached pigmented discs (control 2); G3, bleached untreated discs; G4, bleached pigmented discs. The discs were adapted to artificial pulp chambers, which were placed in wells of 24-well plates containing 1 mL culture medium (Dulbecco’s modified Eagle’s medium [DMEM]). After applying the bleaching gel on enamel, the extracts (DMEM + components of bleaching gel that diffused through the discs) were collected and then applied on the cultured MDPC-23 odontoblast-like cells. Cell viability (methyl tetrazolium assay and Live & Dead, Calcein AM, and ethidium homodimer-1 [EthD-1] probes), the amount of H2O2 that diffused through enamel and dentin (leuco-crystal violet product), and the H2O2-mediated oxidative cell stress (SOx) and components of degradation were assessed (analysis of variance/Tukey; a=0.05). Results: There was no significant difference between the groups G1 and G2 for all the parameters tested (p.0.05). Reduction in the trans-enamel and trans-dentin diffusion of H2O2 occurred for G4 in comparison with G3. Significantly lower cell viability associated with greater oxidative stress was observed for G3 (p,0.05). Therefore, in-office tooth bleaching therapy performed in pigmented samples caused lower cytotoxic effects compared with untreated samples submitted to the same esthetic procedure (p,0.05). Conclusion: According to the methodology used in this investigation, the authors concluded that the presence of pigments in hard tooth structures decreases the trans-enamel and trans-dentin diffusion of H2O2 and the toxicity to pulp cells of an in-office bleaching gel with 35% H2O2. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
Descrição: dc.description | Department of Dental Materials and Prosthodontics Araraquara School of Dentistry University Estadual Paulista–UNESP | - |
Descrição: dc.description | Department of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry University de São Paulo–USP | - |
Descrição: dc.description | Department of Restorative Dentistry Araçatuba School of Dentistry University Estadual Paulista–UNESP | - |
Descrição: dc.description | Department of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry University Estadual Paulista–UNESP | - |
Descrição: dc.description | Department of Physiology and Pathology Araraquara School of Dentistry University Estadual Paulista–UNESP | - |
Descrição: dc.description | Department of Dental Materials and Prosthodontics Araraquara School of Dentistry University Estadual Paulista–UNESP | - |
Descrição: dc.description | Department of Restorative Dentistry Araçatuba School of Dentistry University Estadual Paulista–UNESP | - |
Descrição: dc.description | Department of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry University Estadual Paulista–UNESP | - |
Descrição: dc.description | Department of Physiology and Pathology Araraquara School of Dentistry University Estadual Paulista–UNESP | - |
Descrição: dc.description | FAPESP: 2015/21770-4 | - |
Descrição: dc.description | FAPESP: 2016/10928-9 | - |
Descrição: dc.description | CNPq: 303599/2014-6 | - |
Descrição: dc.description | CNPq: 443153/2014-0 | - |
Formato: dc.format | 632-642 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Operative Dentistry | - |
???dc.source???: dc.source | Scopus | - |
Título: dc.title | Influence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleaching | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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