Influence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleaching

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorde Oliveira Duque, C. C. [UNESP]-
Autor(es): dc.creatorSoares, D. G.-
Autor(es): dc.creatorBriso, A. L.F. [UNESP]-
Autor(es): dc.creatorOrtecho-Zuta, U. [UNESP]-
Autor(es): dc.creatorde Oliveira Ribeiro, R. A. [UNESP]-
Autor(es): dc.creatorHebling, J. [UNESP]-
Autor(es): dc.creatorde Souza Costa, C. A. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:48:20Z-
Data de disponibilização: dc.date.available2022-02-22T00:48:20Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.2341/19-013-L-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206822-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206822-
Descrição: dc.descriptionObjective: The aim of this study was to evaluate the influence of the presence of pigments in tooth structures on the trans-enamel and trans-dentin diffusion of hydrogen peroxide (H2O2) and its cytotoxicity after carrying out an in-office bleaching therapy. Methods and Materials: A bleaching gel with 35% H2O2 was applied for 45 minutes (three times for 15 minutes) on enamel and dentin discs (n=6), either previously submitted to the intrinsic pigmentation protocol with a concentrated solution of black tea, or not, defining the following groups: G1, unbleached untreated discs (control 1); G2, unbleached pigmented discs (control 2); G3, bleached untreated discs; G4, bleached pigmented discs. The discs were adapted to artificial pulp chambers, which were placed in wells of 24-well plates containing 1 mL culture medium (Dulbecco’s modified Eagle’s medium [DMEM]). After applying the bleaching gel on enamel, the extracts (DMEM + components of bleaching gel that diffused through the discs) were collected and then applied on the cultured MDPC-23 odontoblast-like cells. Cell viability (methyl tetrazolium assay and Live & Dead, Calcein AM, and ethidium homodimer-1 [EthD-1] probes), the amount of H2O2 that diffused through enamel and dentin (leuco-crystal violet product), and the H2O2-mediated oxidative cell stress (SOx) and components of degradation were assessed (analysis of variance/Tukey; a=0.05). Results: There was no significant difference between the groups G1 and G2 for all the parameters tested (p.0.05). Reduction in the trans-enamel and trans-dentin diffusion of H2O2 occurred for G4 in comparison with G3. Significantly lower cell viability associated with greater oxidative stress was observed for G3 (p,0.05). Therefore, in-office tooth bleaching therapy performed in pigmented samples caused lower cytotoxic effects compared with untreated samples submitted to the same esthetic procedure (p,0.05). Conclusion: According to the methodology used in this investigation, the authors concluded that the presence of pigments in hard tooth structures decreases the trans-enamel and trans-dentin diffusion of H2O2 and the toxicity to pulp cells of an in-office bleaching gel with 35% H2O2.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry University Estadual Paulista–UNESP-
Descrição: dc.descriptionDepartment of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry University de São Paulo–USP-
Descrição: dc.descriptionDepartment of Restorative Dentistry Araçatuba School of Dentistry University Estadual Paulista–UNESP-
Descrição: dc.descriptionDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry University Estadual Paulista–UNESP-
Descrição: dc.descriptionDepartment of Physiology and Pathology Araraquara School of Dentistry University Estadual Paulista–UNESP-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry University Estadual Paulista–UNESP-
Descrição: dc.descriptionDepartment of Restorative Dentistry Araçatuba School of Dentistry University Estadual Paulista–UNESP-
Descrição: dc.descriptionDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry University Estadual Paulista–UNESP-
Descrição: dc.descriptionDepartment of Physiology and Pathology Araraquara School of Dentistry University Estadual Paulista–UNESP-
Descrição: dc.descriptionFAPESP: 2015/21770-4-
Descrição: dc.descriptionFAPESP: 2016/10928-9-
Descrição: dc.descriptionCNPq: 303599/2014-6-
Descrição: dc.descriptionCNPq: 443153/2014-0-
Formato: dc.format632-642-
Idioma: dc.languageen-
Relação: dc.relationOperative Dentistry-
???dc.source???: dc.sourceScopus-
Título: dc.titleInfluence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleaching-
Tipo de arquivo: dc.typelivro digital-
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