Silencing matrix metalloproteinase-13 (Mmp-13) reduces inflammatory bone resorption associated with LPS-induced periodontal disease in vivo

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorState University of New York-
Autor(es): dc.creatorGuimaraes-Stabili, Morgana R. [UNESP]-
Autor(es): dc.creatorde Medeiros, Marcell Costa [UNESP]-
Autor(es): dc.creatorRossi, Danuza [UNESP]-
Autor(es): dc.creatorCamilli, Angelo Constantino [UNESP]-
Autor(es): dc.creatorZanelli, Cleslei Fernando [UNESP]-
Autor(es): dc.creatorValentini, Sandro Roberto [UNESP]-
Autor(es): dc.creatorSpolidorio, Luis Carlos [UNESP]-
Autor(es): dc.creatorKirkwood, Keith Lough-
Autor(es): dc.creatorRossa, Carlos [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:48:04Z-
Data de disponibilização: dc.date.available2022-02-22T00:48:04Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00784-020-03644-3-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206770-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206770-
Descrição: dc.descriptionObjectives: The aim of this study was to evaluate the effect of specific inhibition of MMP-13 on inflammation and inflammatory bone resorption in a murine model of lipopolysaccharide (LPS)-induced periodontitis. Materials and methods: Periodontitis was induced in mice by micro-injections of LPS into the gingival tissues adjacent to the palatal surfaces of maxillary molars twice a week for 15 days. Matrix metalloproteinase-13 (Mmp-13) shRNA or a specific biochemical inhibitor were also injected into the same sites in alternating days with the LPS injections. Efficacy of shRNA-mediated silencing of Mmp-13 was verified by quantitative real-time polymerase chain reaction (qPCR) and immunoblot. Bone resorption was assessed by microcomputed tomography (uCT). Histological sections stained with hematoxylin/eosin (H/E) were used in the stereometric analysis of the inflammatory infiltrate. Gingival tissues were used to evaluate expression of Mmp-13, Il-6, Tnf-α, Ptgs2, and Rankl (qPCR). Protein levels of TGF-β and IL-10 in the tissues were determined by enzyme-linked immunosorbent assays (ELISA) or by MMP-13 and p38 immunoblot. Results: Silencing Mmp-13 expression reduced bone resorption significantly. Expression of Mmp-13, Il-6, and Tnf-α, as well as the protein levels of IL-6 and TNF-α, was reduced in the animals treated with adenovirus-delivered shRNA; however, these effects were not associated with modulation of p38 MAPK signaling. Interestingly, inhibition Mmp-13 did not affect the severity of inflammatory infiltrate. Conclusions: Site-specific inhibition of MMP-13 reduced bone resorption and production of inflammatory mediators associated with periodontal disease. Clinical relevance: The results suggest that site-specific inhibition of MMP-13 may be an interesting strategy to modulate inflammation and reduce bone resorption in osteolytic inflammatory diseases.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara-State University of São Paulo UNESP, Rua Humaita, 1680, Centro-
Descrição: dc.descriptionDepartment of Biological Sciences School of Pharmaceutical Sciences UNESP-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry at Araraquara UNESP-
Descrição: dc.descriptionDepartment of Oral Biology University at Buffalo State University of New York-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara-State University of São Paulo UNESP, Rua Humaita, 1680, Centro-
Descrição: dc.descriptionDepartment of Biological Sciences School of Pharmaceutical Sciences UNESP-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry at Araraquara UNESP-
Descrição: dc.descriptionFAPESP: 2007/05583-3-
Formato: dc.format3161-3172-
Idioma: dc.languageen-
Relação: dc.relationClinical Oral Investigations-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBone resorption-
Palavras-chave: dc.subjectInflammation-
Palavras-chave: dc.subjectLipopolysaccharide-
Palavras-chave: dc.subjectMetalloproteinase-13-
Palavras-chave: dc.subjectPeriodontal disease-
Título: dc.titleSilencing matrix metalloproteinase-13 (Mmp-13) reduces inflammatory bone resorption associated with LPS-induced periodontal disease in vivo-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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