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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | State University of New York | - |
Autor(es): dc.creator | Guimaraes-Stabili, Morgana R. [UNESP] | - |
Autor(es): dc.creator | de Medeiros, Marcell Costa [UNESP] | - |
Autor(es): dc.creator | Rossi, Danuza [UNESP] | - |
Autor(es): dc.creator | Camilli, Angelo Constantino [UNESP] | - |
Autor(es): dc.creator | Zanelli, Cleslei Fernando [UNESP] | - |
Autor(es): dc.creator | Valentini, Sandro Roberto [UNESP] | - |
Autor(es): dc.creator | Spolidorio, Luis Carlos [UNESP] | - |
Autor(es): dc.creator | Kirkwood, Keith Lough | - |
Autor(es): dc.creator | Rossa, Carlos [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:48:04Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:48:04Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-05-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1007/s00784-020-03644-3 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/206770 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/206770 | - |
Descrição: dc.description | Objectives: The aim of this study was to evaluate the effect of specific inhibition of MMP-13 on inflammation and inflammatory bone resorption in a murine model of lipopolysaccharide (LPS)-induced periodontitis. Materials and methods: Periodontitis was induced in mice by micro-injections of LPS into the gingival tissues adjacent to the palatal surfaces of maxillary molars twice a week for 15 days. Matrix metalloproteinase-13 (Mmp-13) shRNA or a specific biochemical inhibitor were also injected into the same sites in alternating days with the LPS injections. Efficacy of shRNA-mediated silencing of Mmp-13 was verified by quantitative real-time polymerase chain reaction (qPCR) and immunoblot. Bone resorption was assessed by microcomputed tomography (uCT). Histological sections stained with hematoxylin/eosin (H/E) were used in the stereometric analysis of the inflammatory infiltrate. Gingival tissues were used to evaluate expression of Mmp-13, Il-6, Tnf-α, Ptgs2, and Rankl (qPCR). Protein levels of TGF-β and IL-10 in the tissues were determined by enzyme-linked immunosorbent assays (ELISA) or by MMP-13 and p38 immunoblot. Results: Silencing Mmp-13 expression reduced bone resorption significantly. Expression of Mmp-13, Il-6, and Tnf-α, as well as the protein levels of IL-6 and TNF-α, was reduced in the animals treated with adenovirus-delivered shRNA; however, these effects were not associated with modulation of p38 MAPK signaling. Interestingly, inhibition Mmp-13 did not affect the severity of inflammatory infiltrate. Conclusions: Site-specific inhibition of MMP-13 reduced bone resorption and production of inflammatory mediators associated with periodontal disease. Clinical relevance: The results suggest that site-specific inhibition of MMP-13 may be an interesting strategy to modulate inflammation and reduce bone resorption in osteolytic inflammatory diseases. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Department of Diagnosis and Surgery School of Dentistry at Araraquara-State University of São Paulo UNESP, Rua Humaita, 1680, Centro | - |
Descrição: dc.description | Department of Biological Sciences School of Pharmaceutical Sciences UNESP | - |
Descrição: dc.description | Department of Physiology and Pathology School of Dentistry at Araraquara UNESP | - |
Descrição: dc.description | Department of Oral Biology University at Buffalo State University of New York | - |
Descrição: dc.description | Department of Diagnosis and Surgery School of Dentistry at Araraquara-State University of São Paulo UNESP, Rua Humaita, 1680, Centro | - |
Descrição: dc.description | Department of Biological Sciences School of Pharmaceutical Sciences UNESP | - |
Descrição: dc.description | Department of Physiology and Pathology School of Dentistry at Araraquara UNESP | - |
Descrição: dc.description | FAPESP: 2007/05583-3 | - |
Formato: dc.format | 3161-3172 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Clinical Oral Investigations | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Bone resorption | - |
Palavras-chave: dc.subject | Inflammation | - |
Palavras-chave: dc.subject | Lipopolysaccharide | - |
Palavras-chave: dc.subject | Metalloproteinase-13 | - |
Palavras-chave: dc.subject | Periodontal disease | - |
Título: dc.title | Silencing matrix metalloproteinase-13 (Mmp-13) reduces inflammatory bone resorption associated with LPS-induced periodontal disease in vivo | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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