Tempol improves redox status in mdx dystrophic diaphragm muscle

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversity of Brasilia (UnB)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorHermes, Túlio de Almeida-
Autor(es): dc.creatorMizobuti, Daniela Sayuri-
Autor(es): dc.creatorda Rocha, Guilherme Luiz-
Autor(es): dc.creatorda Silva, Heloina Nathalliê Mariano-
Autor(es): dc.creatorCovatti, Caroline-
Autor(es): dc.creatorPereira, Elaine Cristina Leite-
Autor(es): dc.creatorFerretti, Renato [UNESP]-
Autor(es): dc.creatorMinatel, Elaine-
Data de aceite: dc.date.accessioned2022-02-22T00:47:56Z-
Data de disponibilização: dc.date.available2022-02-22T00:47:56Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/iep.12376-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206714-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206714-
Descrição: dc.descriptionOxidative stress is a critical element in relationship to the pathophysiology of Duchenne muscular dystrophy (DMD). In the mice the diaphragm (DIA) is most resembles the dystrophic human pathology. In this study we have evaluated the consequences of a synthetic antioxidant (tempol) on oxidative stress parameters in the DIA muscle of mdx mice. The mdx mice were separated into two groups: mdx, the control group receiving intraperitoneal (i.p.) injections of saline solution (100 µL), and mdxT, the treated group receiving i.p. injections of tempol (100 mg/kg). The tempol-treated group showed reduced oxidative stress markers, decreasing the dihydroethidium reaction (DHE) area; autofluorescent lipofuscin granules; and 4-hydroxynonenal (4-HNE)-protein adduct levels. DIA muscle of mdx mice. At the same time, the manganese-superoxide dismutase 2 (SOD2) levels were increased in the tempol-treated group. In addition, the tempol-treated group showed reduced levels of glutathione-disulphide reductase (GSR), glutathione peroxidase 1 (GPx1) and catalase (CAT) in immunoblots. The tempol-treated group has also shown lower relative gene expression of SOD1, CAT and GPx than the non-treated group. Our data demonstrated that tempol treatment reduced oxidant parameters and increased anti-oxidant SOD2 levels in the DIA muscle of mdx mice, which may contribute to the normalization of the redox homeostasis of dystrophic muscles.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biology State University of Campinas (UNICAMP)-
Descrição: dc.descriptionFaculty of Ceilandia University of Brasilia (UnB)-
Descrição: dc.descriptionDepartment of Anatomy Institute of Bioscience of Botucatu São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Anatomy Institute of Bioscience of Botucatu São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 17/01638-0-
Formato: dc.format289-297-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Experimental Pathology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectdiaphragm muscle-
Palavras-chave: dc.subjectdystrophic muscles-
Palavras-chave: dc.subjectmdx mice-
Palavras-chave: dc.subjectoxidative stress-
Palavras-chave: dc.subjectredox homeostasis-
Título: dc.titleTempol improves redox status in mdx dystrophic diaphragm muscle-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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