Ultradeformable Lipid Vesicles Localize Amphotericin B in the Dermis for the Treatment of Infectious Skin Diseases

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Autor(es): dc.contributorUniversidad Complutense de Madrid-
Autor(es): dc.contributorUniversity of Portsmouth-
Autor(es): dc.contributorUniversity of Saõ Paulo-
Autor(es): dc.contributorUniversidad Ceu Cardenal Herrera-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorFernández-Garciá, Raquel-
Autor(es): dc.creatorStatts, Larry-
Autor(es): dc.creatorDe Jesus, Jéssica A.-
Autor(es): dc.creatorDea-Ayuela, Maria Auxiliadora-
Autor(es): dc.creatorBautista, Liliana-
Autor(es): dc.creatorSimaõ, Rúben-
Autor(es): dc.creatorBolás-Fernández, Francisco-
Autor(es): dc.creatorBallesteros, Maria Paloma-
Autor(es): dc.creatorLaurenti, Marcia Dalastra-
Autor(es): dc.creatorPassero, Luiz F. D. [UNESP]-
Autor(es): dc.creatorLalatsa, Aikaterini-
Autor(es): dc.creatorSerrano, Dolores R.-
Data de aceite: dc.date.accessioned2022-02-22T00:47:53Z-
Data de disponibilização: dc.date.available2022-02-22T00:47:53Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-10-09-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1021/acsinfecdis.0c00293-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206694-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206694-
Descrição: dc.descriptionCutaneous fungal and parasitic diseases remain challenging to treat, as available therapies are unable to permeate the skin barrier. Thus, treatment options rely on systemic therapy, which fail to produce high local drug concentrations but can lead to significant systemic toxicity. Amphotericin B (AmB) is highly efficacious in the treatment of both fungal and parasitic diseases such as cutaneous leishmaniasis but is reserved for parenteral administration in patients with severe pathophysiology. Here, we have designed and optimized AmB-transfersomes [93.5% encapsulation efficiency, 150 nm size, and good colloidal stability (-35.02 mV)] that can remain physicochemically stable (>90% drug content) at room temperature and 4 °C over 6 months when lyophilized and stored under desiccated conditions. AmB-transfersomes possessed good permeability across mouse skin (4.91 ± 0.41 μg/cm2/h) and 10-fold higher permeability across synthetic Strat-M membranes. In vivo studies after a single topical application in mice showed permeability and accumulation within the dermis (>25 μg AmB/g skin 6 h postadministration), indicating the delivery of therapeutic amounts of AmB for mycoses and cutaneous leishmaniasis, while a single daily administration in Leishmania (Leishmania) amazonensis infected mice over 10 days, resulted in excellent efficacy (98% reduction in Leishmania parasites). Combining the application of AmB-transfersomes with metallic microneedles in vivo increased the levels in the SC and dermis but was unlikely to elicit transdermal levels. In conclusion, AmB-transfersomes are promising and stable topical nanomedicines that can be readily translated for parasitic and fungal infectious diseases.-
Descrição: dc.descriptionDepartamento de Farmacia Galénica y Tecnologiá Alimentaria Departamento de Microbiologiá y Parasitologiá Instituto Universitario de Farmacia Industrial Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n-
Descrição: dc.descriptionBiomaterials Bio-engineering and Nanomedicines (BioN) Laboratory Institute of Biomedical and Biomolecular Sciences School of Pharmacy and Pharmaceutical Sciences University of Portsmouth, St. Michael's Building, White Swan Road-
Descrição: dc.descriptionLaboratory of Pathology of Infectious Diseases (LIM-50) Medical School University of Saõ Paulo, Avenida Dr. Arnaldo 455-
Descrição: dc.descriptionDepartamento de Farmacia Universidad Ceu Cardenal Herrera, Carrer Santiago Ramón y Cajal s/n-
Descrição: dc.descriptionSaõ Paulo State University (UNESP) Institute of Biosciences, Saõ Vicente Pracą Infante Dom Henrique s/n-
Descrição: dc.descriptionSaõ Paulo State University (UNESP) Institute for Advanced Studies of Ocean, Saõ Vicente Av. Joaõ Francisco Bensdorp 1178-
Descrição: dc.descriptionSaõ Paulo State University (UNESP) Institute of Biosciences, Saõ Vicente Pracą Infante Dom Henrique s/n-
Descrição: dc.descriptionSaõ Paulo State University (UNESP) Institute for Advanced Studies of Ocean, Saõ Vicente Av. Joaõ Francisco Bensdorp 1178-
Formato: dc.format2647-2660-
Idioma: dc.languageen-
Relação: dc.relationACS Infectious Diseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectamphotericin B-
Palavras-chave: dc.subjectfungal infections-
Palavras-chave: dc.subjectleishmaniasis-
Palavras-chave: dc.subjecttape stripping-
Palavras-chave: dc.subjecttransfersomes-
Palavras-chave: dc.subjectultradeformable lipid vesicles-
Título: dc.titleUltradeformable Lipid Vesicles Localize Amphotericin B in the Dermis for the Treatment of Infectious Skin Diseases-
Tipo de arquivo: dc.typelivro digital-
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