Novel colloidal nanocarrier of cetylpyridinium chloride: Antifungal activities on candida species and cytotoxic potential on murine fibroblasts

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Western São Paulo (UNOESTE)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorAraujo, Heitor Ceolin [UNESP]-
Autor(es): dc.creatorArias, Laís Salomão [UNESP]-
Autor(es): dc.creatorMorais Caldeirão, Anne Caroline-
Autor(es): dc.creatorDe Freitas Assumpção, Lanay Caroline-
Autor(es): dc.creatorMorceli, Marcela Grigoletto-
Autor(es): dc.creatorDe Souza Neto, Francisco Nunes [UNESP]-
Autor(es): dc.creatorDe Camargo, Emerson Rodrigues-
Autor(es): dc.creatorOliveira, Sandra Helena Penha [UNESP]-
Autor(es): dc.creatorPessan, Juliano Pelim [UNESP]-
Autor(es): dc.creatorMonteiro, Douglas Roberto-
Data de aceite: dc.date.accessioned2022-02-22T00:47:48Z-
Data de disponibilização: dc.date.available2022-02-22T00:47:48Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/jof6040218-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206673-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206673-
Descrição: dc.descriptionNanocarriers have been used as alternative tools to overcome the resistance of Candida species to conventional treatments. This study prepared a nanocarrier of cetylpyridinium chloride (CPC) using iron oxide nanoparticles (IONPs) conjugated with chitosan (CS), and assessed its antifungal and cytotoxic effects. CPC was immobilized on CS-coated IONPs, and the nanocarrier was physico-chemically characterized. Antifungal effects were determined on planktonic cells of Candida albicans and Candida glabrata (by minimum inhibitory concentration (MIC) assays) and on single- and dual-species biofilms of these strains (by quantification of cultivable cells, total biomass and metabolic activity). Murine fibroblasts were exposed to different concentrations of the nanocarrier, and the cytotoxic effect was evaluated by MTT reduction assay. Characterization methods confirmed the presence of a nanocarrier smaller than 313 nm. IONPs-CS-CPC and free CPC showed the same MIC values (0.78 µg mL−1). CPC-containing nanocarrier at 78 µg mL−1 significantly reduced the number of cultivable cells for all biofilms, surpassing the effect promoted by free CPC. For total biomass, metabolic activity, and cytotoxic effects, the nanocarrier and free CPC produced statistically similar outcomes. In conclusion, the IONPs-CS-CPC nanocarrier was more effective than CPC in reducing the cultivable cells of Candida biofilms without increasing the cytotoxic effects of CPC, and may be a useful tool for the treatment of oral fungal infections.-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP)-
Descrição: dc.descriptionUniversity of Western São Paulo (UNOESTE)-
Descrição: dc.descriptionSchool of Dentistry University of Western São Paulo (UNOESTE)-
Descrição: dc.descriptionDepartment of Chemistry Federal University of São Carlos (UFSCar)-
Descrição: dc.descriptionDepartment of Basic Sciences School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Basic Sciences School of Dentistry São Paulo State University (UNESP)-
Formato: dc.format1-14-
Idioma: dc.languageen-
Relação: dc.relationJournal of Fungi-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntifungal agents-
Palavras-chave: dc.subjectBiofilms-
Palavras-chave: dc.subjectCandida albicans-
Palavras-chave: dc.subjectCandida glabrata-
Palavras-chave: dc.subjectCetylpyridinium chloride-
Palavras-chave: dc.subjectChitosan-
Palavras-chave: dc.subjectIron oxide nanoparticles-
Título: dc.titleNovel colloidal nanocarrier of cetylpyridinium chloride: Antifungal activities on candida species and cytotoxic potential on murine fibroblasts-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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