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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade de São Paulo (USP) | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | São Carlos Federal University | - |
Autor(es): dc.creator | Carvalho, Thamyris Souza | - |
Autor(es): dc.creator | Araújo, Tamara Teodoro | - |
Autor(es): dc.creator | Ventura, Talita Mendes Oliveira | - |
Autor(es): dc.creator | Dionizio, Aline | - |
Autor(es): dc.creator | Câmara, João Victor Frazão | - |
Autor(es): dc.creator | Moraes, Samanta Mascarenhas | - |
Autor(es): dc.creator | Pelá, Vinicius Taioqui | - |
Autor(es): dc.creator | Martini, Tatiana | - |
Autor(es): dc.creator | Leme, Julia Chaparro | - |
Autor(es): dc.creator | Derbotolli, Ana Luiza Bogaz | - |
Autor(es): dc.creator | Grizzo, Larissa Tercilia | - |
Autor(es): dc.creator | Crusca, Edson [UNESP] | - |
Autor(es): dc.creator | Shibao, Priscila Yumi Tanaka | - |
Autor(es): dc.creator | Marchetto, Reinaldo [UNESP] | - |
Autor(es): dc.creator | Henrique-Silva, Flavio | - |
Autor(es): dc.creator | Pessan, Juliano Pelim [UNESP] | - |
Autor(es): dc.creator | Buzalaf, Marília Afonso Rabelo | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:47:37Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:47:37Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2020-10-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1016/j.jdent.2020.103478 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/206608 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/206608 | - |
Descrição: dc.description | Objectives: To evaluate, in vivo: 1) proteomic alterations in the acquired enamel pellicle (AEP) after treatment with sugarcane-derived cystatin (CaneCPI-5), hemoglobin (HB), statherin-derived peptide (StN15) or their combination before the formation of the AEP and subsequent erosive challenge; 2) the protection of these treatments against erosive demnineralization. Materials and methods: In 5 crossover phases, after prophylaxis, 10 volunteers rinsed (10 mL, 1 min) with: deionized water-1, 0.1 mg/mL CaneCPI-5-2, 1.0 mg/mL HB-3, 1.88 × 10-5 M StN15-4 or their combination-5. AEP was formed (2 h) and enamel biopsy (10 μL, 1%citric acid, pH 2.5, 10 s) was performed on one incisor for calcium analysis. The same acid was applied on the vestibular surfaces of the remaining teeth. The acid-resistant proteins within the remaining AEP were collected. Samples were quantitatively analyzed by label-free proteomics. Results: Treatment with the proteins/peptide, isolated or combined, increased several acid-resistant proteins in the AEP, compared with control. The highest increases were seen for PRPs (32-fold, StN15), profilin (15-fold, combination), alpha-amylase (9-fold; StN15), keratins (8-fold, CaneCPI-5 and HB), Histatin-1 (7-fold, StN15), immunoglobulins (6.5-fold, StN15), lactotransferrin (4-fold, CaneCPI-5), cystatins, lysozyme, protein S-100-A9 and actins (3.5-fold, StN15), serum albumin (3.5-fold, CaneCPI-5 and HB) and hemoglobin (3-fold, StN15). Annexin, calmodulin, keratin, tubulin and cystatins were identified exclusively upon treatment with the proteins/peptide, alone or combined. Groups 2, 3 and 4 had significantly lower Ca released from enamel compared to group 1 (Kruskal-Wallis/Dunn's, p < 0.05). Conclusions: Treatment with CaneCPI-5, HB or StN15 remarkably increases acid-resistant proteins in the AEP, protecting against erosion. Clinical significance: Our results show, for the first time, that treatment with proteins/peptide remarkably increases acid-resistant proteins in the AEP, protecting against erosive demineralization. These findings open an avenue for a new preventive approach for erosive demineralization, employing acquired pellicle engineering procedures that may in the future be incorporated into dental products. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
Descrição: dc.description | Department of Biological Sciences Bauru School of Dentistry University of São Paulo | - |
Descrição: dc.description | Department of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Genetics and Evolution São Carlos Federal University | - |
Descrição: dc.description | Department of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP) | - |
Descrição: dc.description | FAPESP: 2018/12041-7 | - |
Descrição: dc.description | FAPESP: 2019/16254-8 | - |
Descrição: dc.description | CNPq: 407853/2018-9 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Journal of Dentistry | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Acquired enamel pellicle | - |
Palavras-chave: dc.subject | Cystatin | - |
Palavras-chave: dc.subject | Dental erosion | - |
Palavras-chave: dc.subject | Hemoglobin | - |
Palavras-chave: dc.subject | Proteomics | - |
Palavras-chave: dc.subject | Statherin | - |
Título: dc.title | Acquired pellicle protein-based engineering protects against erosive demineralization | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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