Acquired pellicle protein-based engineering protects against erosive demineralization

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorSão Carlos Federal University-
Autor(es): dc.creatorCarvalho, Thamyris Souza-
Autor(es): dc.creatorAraújo, Tamara Teodoro-
Autor(es): dc.creatorVentura, Talita Mendes Oliveira-
Autor(es): dc.creatorDionizio, Aline-
Autor(es): dc.creatorCâmara, João Victor Frazão-
Autor(es): dc.creatorMoraes, Samanta Mascarenhas-
Autor(es): dc.creatorPelá, Vinicius Taioqui-
Autor(es): dc.creatorMartini, Tatiana-
Autor(es): dc.creatorLeme, Julia Chaparro-
Autor(es): dc.creatorDerbotolli, Ana Luiza Bogaz-
Autor(es): dc.creatorGrizzo, Larissa Tercilia-
Autor(es): dc.creatorCrusca, Edson [UNESP]-
Autor(es): dc.creatorShibao, Priscila Yumi Tanaka-
Autor(es): dc.creatorMarchetto, Reinaldo [UNESP]-
Autor(es): dc.creatorHenrique-Silva, Flavio-
Autor(es): dc.creatorPessan, Juliano Pelim [UNESP]-
Autor(es): dc.creatorBuzalaf, Marília Afonso Rabelo-
Data de aceite: dc.date.accessioned2022-02-22T00:47:37Z-
Data de disponibilização: dc.date.available2022-02-22T00:47:37Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2020.103478-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206608-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206608-
Descrição: dc.descriptionObjectives: To evaluate, in vivo: 1) proteomic alterations in the acquired enamel pellicle (AEP) after treatment with sugarcane-derived cystatin (CaneCPI-5), hemoglobin (HB), statherin-derived peptide (StN15) or their combination before the formation of the AEP and subsequent erosive challenge; 2) the protection of these treatments against erosive demnineralization. Materials and methods: In 5 crossover phases, after prophylaxis, 10 volunteers rinsed (10 mL, 1 min) with: deionized water-1, 0.1 mg/mL CaneCPI-5-2, 1.0 mg/mL HB-3, 1.88 × 10-5 M StN15-4 or their combination-5. AEP was formed (2 h) and enamel biopsy (10 μL, 1%citric acid, pH 2.5, 10 s) was performed on one incisor for calcium analysis. The same acid was applied on the vestibular surfaces of the remaining teeth. The acid-resistant proteins within the remaining AEP were collected. Samples were quantitatively analyzed by label-free proteomics. Results: Treatment with the proteins/peptide, isolated or combined, increased several acid-resistant proteins in the AEP, compared with control. The highest increases were seen for PRPs (32-fold, StN15), profilin (15-fold, combination), alpha-amylase (9-fold; StN15), keratins (8-fold, CaneCPI-5 and HB), Histatin-1 (7-fold, StN15), immunoglobulins (6.5-fold, StN15), lactotransferrin (4-fold, CaneCPI-5), cystatins, lysozyme, protein S-100-A9 and actins (3.5-fold, StN15), serum albumin (3.5-fold, CaneCPI-5 and HB) and hemoglobin (3-fold, StN15). Annexin, calmodulin, keratin, tubulin and cystatins were identified exclusively upon treatment with the proteins/peptide, alone or combined. Groups 2, 3 and 4 had significantly lower Ca released from enamel compared to group 1 (Kruskal-Wallis/Dunn's, p < 0.05). Conclusions: Treatment with CaneCPI-5, HB or StN15 remarkably increases acid-resistant proteins in the AEP, protecting against erosion. Clinical significance: Our results show, for the first time, that treatment with proteins/peptide remarkably increases acid-resistant proteins in the AEP, protecting against erosive demineralization. These findings open an avenue for a new preventive approach for erosive demineralization, employing acquired pellicle engineering procedures that may in the future be incorporated into dental products.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo-
Descrição: dc.descriptionDepartment of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Genetics and Evolution São Carlos Federal University-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2018/12041-7-
Descrição: dc.descriptionFAPESP: 2019/16254-8-
Descrição: dc.descriptionCNPq: 407853/2018-9-
Idioma: dc.languageen-
Relação: dc.relationJournal of Dentistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAcquired enamel pellicle-
Palavras-chave: dc.subjectCystatin-
Palavras-chave: dc.subjectDental erosion-
Palavras-chave: dc.subjectHemoglobin-
Palavras-chave: dc.subjectProteomics-
Palavras-chave: dc.subjectStatherin-
Título: dc.titleAcquired pellicle protein-based engineering protects against erosive demineralization-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.