Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) | - |
Autor(es): dc.contributor | Universidade Federal de São Carlos (UFSCar) | - |
Autor(es): dc.creator | Wong, Ademar [UNESP] | - |
Autor(es): dc.creator | Santos, Anderson M. | - |
Autor(es): dc.creator | Fatibello-Filho, Orlando | - |
Autor(es): dc.creator | Sotomayor, Maria D. P. T. [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:47:33Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:47:33Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-01-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1002/elan.202060084 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/206583 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/206583 | - |
Descrição: dc.description | In this work, a renewable tyrosinase-based biosensor was developed for the detection of catechol, using a carbon black paste electrode, without any mediator. The effect of pH, type of electrolyte, and amount of tyrosinase enzyme were explored for optimum analytical performance. The best-performing biosensor in amperometric experiments at potential −0.2 V vs. Ag/AgCl (3 mol L−1 KCl) was obtained using a 0.1 mol L−1 phosphate buffer solution (pH 7.0) as electrolyte. Under optimized conditions, the proposed biosensor had two concentration linear ranges from 5.0×10−9 to 4.8×10−8 and from 4.8×10−8 to 8.5×10−6 mol L−1 and a limit of detection of 1.5×10−9 mol L−1. The apparent Michaelis-Menten constant ((Formula presented.)) was calculated by the amperometric method, and the obtained value was 1.2×10−5 mol L−1 whose result was similar when compared with other studies previously. The biosensor was applied in river water samples, and the results were very satisfactory, with recoveries near 100 %. In addition, the response of this biosensor for different compounds, taking into account their molecular structures was investigated and the results obtained showed no interference with the response potential of catechol. The electrochemical biosensor developed in this work can be considered highly advantageous because it does not require the use of a mediator (direct detection) for electrochemical response, and also because it is based on a low-cost materials that can be used with success to immobilise other enzymes and/or biomolecules. | - |
Descrição: dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
Descrição: dc.description | Department of Analytical Chemistry Institute of Chemistry State University of São Paulo (UNESP) | - |
Descrição: dc.description | National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) | - |
Descrição: dc.description | Department of Chemistry Federal University of São Carlos, Rod. Washington Luís km 235 | - |
Descrição: dc.description | Department of Analytical Chemistry Institute of Chemistry State University of São Paulo (UNESP) | - |
Descrição: dc.description | CNPq: 405546/2018-1 | - |
Descrição: dc.description | CNPq: 465571/2014-0 | - |
Formato: dc.format | 431-437 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Electroanalysis | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | carbon black | - |
Palavras-chave: dc.subject | catechol | - |
Palavras-chave: dc.subject | electrochemical biosensor | - |
Palavras-chave: dc.subject | glutaraldehyde | - |
Palavras-chave: dc.subject | tyrosinase enzyme | - |
Título: dc.title | Amperometric Tyrosinase Biosensor Based on Carbon Black Paste Electrode for Sensitive Detection of Catechol in Environmental Samples | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: