Comparison of microRNA Expression Profile in Chronic Myeloid Leukemia Patients Newly Diagnosed and Treated by Allogeneic Hematopoietic Stem Cell Transplantation

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorMartins, Juliana Ravelli Baldassarre [UNESP]-
Autor(es): dc.creatorMoraes, Leonardo Nazario de [UNESP]-
Autor(es): dc.creatorCury, Sarah Santiloni [UNESP]-
Autor(es): dc.creatorDadalto, Juliane [UNESP]-
Autor(es): dc.creatorCapannacci, Juliana [UNESP]-
Autor(es): dc.creatorCarvalho, Robson Francisco [UNESP]-
Autor(es): dc.creatorNogueira, Célia Regina [UNESP]-
Autor(es): dc.creatorHokama, Newton Key [UNESP]-
Autor(es): dc.creatorHokama, Paula de Oliveira Montandon [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:47:23Z-
Data de disponibilização: dc.date.available2022-02-22T00:47:23Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-09-04-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fonc.2020.01544-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206531-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206531-
Descrição: dc.descriptionChronic myeloid leukemia (CML) results from a translocation between chromosomes 9 and 22, which generates the Philadelphia chromosome. This forms BCR/ABL1, an active tyrosine kinase protein that promotes cell growth and replication. Despite great progress in CML treatment in the form of tyrosine kinase inhibitors, allogeneic-hematopoietic stem cell transplantation (allo-HSCT) is currently used as an important treatment alternative for patients resistant to these inhibitors. Studies have shown that unregulated expression of microRNAs, which act as oncogenes or tumor suppressors, is associated with human cancers. This contributes to tumor formation and development by stimulating proliferation, angiogenesis, and invasion. Research has demonstrated the potential of microRNAs as biomarkers for cancer diagnosis, prognosis, and therapeutic targets. In the present study, we compared the circulating microRNA expression profiles of 14 newly diagnosed patients with chronic phase-CML and 14 Philadelphia chromosome-negative patients after allo-HSCT. For each patient, we tested 758 microRNAs by reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis. The global expression profile of microRNAs revealed 16 upregulated and 30 downregulated microRNAs. Target genes were analyzed, and key pathways were extracted and compared. Bioinformatics tools were used to analyze data. Among the downregulated miRNA target genes, some genes related to cell proliferation pathways were identified. These results reveal the comprehensive microRNA profile of CML patients and the main pathways related to the target genes of these miRNAs in cytogenetic remission after allo-HSCT. These results provide new resources for exploring stem cell transplantation-based CML treatment strategies.-
Descrição: dc.descriptionDepartment of Internal Medicine São Paulo State University (UNESP-FMB)-
Descrição: dc.descriptionDepartment of Bioprocesses and Biotechnology São Paulo State University (UNESP-FCA)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology São Paulo State University (UNESP-IBB)-
Descrição: dc.descriptionDepartment of Internal Medicine São Paulo State University (UNESP-FMB)-
Descrição: dc.descriptionDepartment of Bioprocesses and Biotechnology São Paulo State University (UNESP-FCA)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology São Paulo State University (UNESP-IBB)-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Oncology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectallogeneic hematopoietic stem cell transplantation-
Palavras-chave: dc.subjectbiomarkers-
Palavras-chave: dc.subjectchronic myeloid leukemia-
Palavras-chave: dc.subjectmiRNAs-
Palavras-chave: dc.subjectPhiladelphia chromosome-
Título: dc.titleComparison of microRNA Expression Profile in Chronic Myeloid Leukemia Patients Newly Diagnosed and Treated by Allogeneic Hematopoietic Stem Cell Transplantation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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