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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Federal University of ABC | - |
Autor(es): dc.contributor | Universidade de São Paulo (USP) | - |
Autor(es): dc.contributor | Université Laval | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Universidade Federal do ABC (UFABC) | - |
Autor(es): dc.contributor | University of California Davis | - |
Autor(es): dc.creator | Ispada, Jessica | - |
Autor(es): dc.creator | da Fonseca Junior, Aldcejam Martins | - |
Autor(es): dc.creator | de Lima, Camila Bruna | - |
Autor(es): dc.creator | Dos Santos, Erika Cristina | - |
Autor(es): dc.creator | Fontes, Patricia Kubo [UNESP] | - |
Autor(es): dc.creator | Nogueira, Marcelo Fábio Gouveia [UNESP] | - |
Autor(es): dc.creator | da Silva, Vinicius Lourenço | - |
Autor(es): dc.creator | Almeida, Fernanda Nascimento | - |
Autor(es): dc.creator | Leite, Saul de Castro | - |
Autor(es): dc.creator | Chitwood, James Lee | - |
Autor(es): dc.creator | Ross, Pablo Juan | - |
Autor(es): dc.creator | Milazzotto, Marcella Pecora | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:47:21Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:47:21Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2020-09-02 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.3390/ijms21186868 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/206516 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/206516 | - |
Descrição: dc.description | In many cell types, epigenetic changes are partially regulated by the availability of metabolites involved in the activity of chromatin-modifying enzymes. Even so, the association between metabolism and the typical epigenetic reprogramming that occurs during preimplantation embryo development remains poorly understood. In this work, we explore the link between energy metabolism, more specifically the tricarboxylic acid cycle (TCA), and epigenetic regulation in bovine preimplantation embryos. Using a morphokinetics model of embryonic development (fast-and slow-developing embryos), we show that DNA methylation (5mC) and hydroxymethylation (5hmC) are dynamically regulated and altered by the speed of the first cleavages. More specifically, slow-developing embryos fail to perform the typical reprogramming that is necessary to ensure the generation of blastocysts with higher ability to establish specific cell lineages. Transcriptome analysis revealed that such differences were mainly associated with enzymes involved in the TCA cycle rather than specific writers/erasers of DNA methylation marks. This relationship was later confirmed by disturbing the embryonic metabolism through changes in α-ketoglutarate or succinate availability in culture media. This was sufficient to interfere with the DNA methylation dynamics despite the fact that blastocyst rates and total cell number were not quite affected. These results provide the first evidence of a relationship between epigenetic reprogramming and energy metabolism in bovine embryos. Likewise, levels of metabolites in culture media may be crucial for precise epigenetic reprogramming, with possible further consequences in the molecular control and differentiation of cells. | - |
Descrição: dc.description | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Laboratory of Embryonic Metabolism and Epigenetics Center of Natural and Human Sciences Federal University of ABC | - |
Descrição: dc.description | Institute of Biomedical Sciences University of Sao Paulo | - |
Descrição: dc.description | Centre de Recherche en Reproduction Développement et Santé Intergénérationnelle (CRDSI) Département des Sciences Animales Faculté des Sciences de l’Agriculture et de l’Alimentation Université Laval | - |
Descrição: dc.description | Laboratory of Phytomedicines Pharmacology and Biotechnology Department of Pharmacology Institute of Biosciences São Paulo State University (Unesp), Campus of Botucatu | - |
Descrição: dc.description | Department of Biological Sciences School of Sciences and Languages São Paulo State University (Unesp), Campus of Assis | - |
Descrição: dc.description | Bioinformatics and Health Informatics Group Center for Engineering Modeling and Applied Social Sciences Universidade Federal do ABC | - |
Descrição: dc.description | Center for Mathematics Computation and Cognition Universidade Federal do ABC | - |
Descrição: dc.description | Department of Animal Science University of California Davis | - |
Descrição: dc.description | Laboratory of Phytomedicines Pharmacology and Biotechnology Department of Pharmacology Institute of Biosciences São Paulo State University (Unesp), Campus of Botucatu | - |
Descrição: dc.description | Department of Biological Sciences School of Sciences and Languages São Paulo State University (Unesp), Campus of Assis | - |
Descrição: dc.description | FAPESP: 2012/50533-2 | - |
Descrição: dc.description | FAPESP: 2015/03381-0 | - |
Descrição: dc.description | FAPESP: 2017/18384-0 | - |
Descrição: dc.description | FAPESP: 2018/11668-6 | - |
Formato: dc.format | 1-16 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | International Journal of Molecular Sciences | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Bovine | - |
Palavras-chave: dc.subject | DNA methylation | - |
Palavras-chave: dc.subject | Embryo | - |
Palavras-chave: dc.subject | Epigenetic | - |
Palavras-chave: dc.subject | Metabolism | - |
Título: dc.title | Tricarboxylic acid cycle metabolites as mediators of dna methylation reprogramming in bovine preimplantation embryos | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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