Semi-quantitative evaluation of Babesia bovis and B. bigemina infection levels estimated by HRM analysis

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorRua Heitor Penteado-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorGiglioti, Rodrigo-
Autor(es): dc.creatorOkino, Cintia Hiromi-
Autor(es): dc.creatorAzevedo, Bianca Tainá-
Autor(es): dc.creatorWedy, Bruna Costa Rodrigues-
Autor(es): dc.creatorGutmanis, Gunta-
Autor(es): dc.creatorVeríssimo, Cecília José-
Autor(es): dc.creatorKatiki, Luciana Morita-
Autor(es): dc.creatorFilho, Anibal Eugênio Vercesi-
Autor(es): dc.creatorde Oliveira, Henrique Nunes [UNESP]-
Autor(es): dc.creatorOliveira, Márcia Cristina de Sena-
Data de aceite: dc.date.accessioned2022-02-22T00:47:09Z-
Data de disponibilização: dc.date.available2022-02-22T00:47:09Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ttbdis.2021.101753-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206466-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206466-
Descrição: dc.descriptionBovine babesiosis is economically the most important arthropod-borne disease of cattle worldwide. The most significant damage caused by bovine babesiosis is attributed to Babesia bovis due to its higher pathogenicity. This study aimed to develop a real-time PCR method followed by HRM (high-resolution melting) analysis for the simultaneous detection of B. bovis and B. bigemina, enabling a semi-quantitative analysis of Babesia levels using a single-tube reaction. The HRM was compared with real-time PCR using species-specific hydrolysis probes. The HRM analysis allowed to differentiate both Babesia species and was sensitive in the detection and differentiation of 10% for each Babesia species in the sample. Our results suggest the use of this method to estimate the prevalence of infections by B. bovis or B. bigemina as an alternative to the methods of absolute quantification by real-time PCR since it neither requires precise estimates of the number of DNA loads nor the construction of calibration curves. The simultaneous detection of the two Babesia species can be used to characterise the infection levels in cattle populations from different geographical regions, allowing a better control of these diseases.-
Descrição: dc.descriptionNational Institute of Animal Science-
Descrição: dc.descriptionInstituto de Zootecnia Rua Heitor Penteado, n. 56, Nova Odessa-
Descrição: dc.descriptionEmbrapa Pecuária Sudeste-
Descrição: dc.descriptionUniversidade Estadual Júlio de Mesquita Filho-
Descrição: dc.descriptionUniversidade Estadual Júlio de Mesquita Filho-
Idioma: dc.languageen-
Relação: dc.relationTicks and Tick-borne Diseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBabesiosis-
Palavras-chave: dc.subjectHRM analysis-
Palavras-chave: dc.subjectqPCR-
Palavras-chave: dc.subjectsemi-quantification-
Título: dc.titleSemi-quantitative evaluation of Babesia bovis and B. bigemina infection levels estimated by HRM analysis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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