Cryopreservation of agronomic plant germplasm using vitrification‐based methods: An overview of selected case studies

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUTP University of Science and Technology in Bydgoszcz-
Autor(es): dc.contributorIslamic Azad University-
Autor(es): dc.creatorRoque‐borda, Cesar Augusto [UNESP]-
Autor(es): dc.creatorKulus, Dariusz-
Autor(es): dc.creatorde Souza, Angela Vacaro [UNESP]-
Autor(es): dc.creatorKaviani, Behzad-
Autor(es): dc.creatorVicente, Eduardo Festozo [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:47:05Z-
Data de disponibilização: dc.date.available2022-02-22T00:47:05Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ijms22116157-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206437-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206437-
Descrição: dc.descriptionNumerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their preservation is imperative for the sake of future breeding programs. Gene banks have been created worldwide to conserve different plant species of cultural and economic importance. Many of them apply cryopreservation, a conservation method in which ultra‐low temperatures (−135 °C to −196 °C) are used for long‐term storage of tissue samples, with little risk of variation occurrence. Cells can be successfully cryopreserved in liquid nitrogen (LN) when the adverse effect of ice crystal formation and growth is mitigated by the removal of water and the formation of the so‐called biological glass (vitrification). This state can be achieved in several ways. The involvement of key cold‐regulated genes and proteins in the acquisition of cold tolerance in plant tissues may additionally improve the survival of LN‐stored explants. The present review explains the importance of cryostorage in agronomy and presents an overview of the recent works accomplished with this strategy. The most widely used cryopreservation techniques, classic and modern cryoprotective agents, and some protocols applied in crops are considered to understand which parameters provide the establishment of high quality and broadly applicable cryopreservation. Attention is also focused on the issues of genetic integrity and functional genomics in plant cryobiology.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionNarodowym Centrum Nauki-
Descrição: dc.descriptionSchool of Agricultural and Veterinarian Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionLaboratory of Ornamental Plants and Vegetable Crops Faculty of Agriculture and Biotechnology UTP University of Science and Technology in Bydgoszcz, Bernardyńska 6-
Descrição: dc.descriptionSchool of Sciences and Engineering São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Horticultural Science Rasht Branch Islamic Azad University-
Descrição: dc.descriptionSchool of Agricultural and Veterinarian Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Sciences and Engineering São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2016/00446‐7-
Descrição: dc.descriptionFAPESP: 2018/25707‐3-
Descrição: dc.descriptionNarodowym Centrum Nauki: 2020/39/D/NZ9/01592-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Molecular Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCryoprotectant-
Palavras-chave: dc.subjectDroplet‐vitrification-
Palavras-chave: dc.subjectEncapsulation-vitrification-
Palavras-chave: dc.subjectEncapsulation‐dehydration-
Palavras-chave: dc.subjectGene banks-
Palavras-chave: dc.subjectGenetic integrity-
Palavras-chave: dc.subjectMolecular markers-
Palavras-chave: dc.subjectSomaclonal variation-
Palavras-chave: dc.subjectVitrification-
Título: dc.titleCryopreservation of agronomic plant germplasm using vitrification‐based methods: An overview of selected case studies-
Aparece nas coleções:Repositório Institucional - Unesp

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