Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorCentro Seccional de Investigación (CIUL)-
Autor(es): dc.contributorUniversidad de Cartagena-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorJaramillo-Lanchero, Rubén D-
Autor(es): dc.creatorSuarez-Alvarez, Paola-
Autor(es): dc.creatorTeheran-Sierra, Luis [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:46:50Z-
Data de disponibilização: dc.date.available2022-02-22T00:46:50Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-04-26-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-021-88388-0-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206346-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206346-
Descrição: dc.descriptionThe effects of respiratory inhibitors, quinone analogues and artificial substrates on the membrane-bound electron transport system of the fastidious β-proteobacterium Eikenella corrodens grown under O2-limited conditions were studied. NADH respiration in isolated membrane particles were partially inhibited by rotenone, dicoumarol, quinacrine, flavone, and capsaicin. A similar response was obtained when succinate oxidation was performed in the presence of thenoyltrifluoroacetone and N,N'-dicyclohexylcarbodiimide. NADH respiration was resistant to site II inhibitors and cyanide, indicating that a percentage of the electrons transported can reach O2 without the bc1 complex. Succinate respiration was sensitive to myxothiazol, antimycin A and 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO). Juglone, plumbagin and menadione had higher reactivity with NADH dehydrogenase. The membrane particles showed the highest oxidase activities with ascorbate-TCHQ (tetrachlorohydroquinone), TCHQ alone, and NADH-TMPD (N,N,N',N'-tetramethyl-p-phenylenediamine), and minor activity levels with ascorbate-DCPIP (2,6-dichloro-phenolindophenol) and NADH-DCPIP. The substrates NADH-DCPIP, NADH-TMPD and TCHQ were electron donors to cyanide-sensitive cbb' cytochrome c oxidase. The presence of dissimilatory nitrate reductase in the aerobic respiratory system of E. corrodens ATCC 23834 was demonstrated by first time. Our results indicate that complexes I and II have resistance to their classic inhibitors, that the oxidation of NADH is stimulated by juglone, plumbagin and menadione, and that sensitivity to KCN is stimulated by the substrates TCHQ, NADH-DCPIP and NADH-TMPD.-
Descrição: dc.descriptionGrupo de Investigación de Biomembranas (GIBIOM) Facultad Ciencias de La Salud Centro Seccional de Investigación (CIUL), Universidad Libre Campus Barranquilla-
Descrição: dc.descriptionGrupo de Micología Departamento de Microbiología Facultad de Medicina Universidad de Cartagena Campus Zaragocilla-
Descrição: dc.descriptionSchool of Agricultural and Veterinary Sciences Technology Department São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n-
Descrição: dc.descriptionSchool of Agricultural and Veterinary Sciences Technology Department São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n-
Formato: dc.format8987-
Idioma: dc.languageen-
Relação: dc.relationScientific reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleEffect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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