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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Fernandes, Carlos A. H. [UNESP] | - |
Autor(es): dc.creator | Morea, Edna G. O. [UNESP] | - |
Autor(es): dc.creator | Cano, Maria Isabel N. [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:46:28Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:46:28Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2020-12-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1007/978-1-0716-1290-3_10 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/206210 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/206210 | - |
Descrição: dc.description | RPA is a conserved heterotrimeric complex and the major single-stranded DNA (ssDNA)-binding protein heterotrimeric complex, which in eukaryotes is formed by the RPA-1, RPA-2, and RPA-3 subunits. The main structural feature of RPA is the presence of the oligonucleotide/oligosaccharide-binding fold (OB-fold) domains, responsible for ssDNA binding and protein:protein interactions. Among the RPA subunits, RPA-1 bears three of the four OB folds involved with RPA-ssDNA binding, although in some organisms RPA-2 can also bind ssDNA. The OB-fold domains are also present in telomere end-binding proteins (TEBP), essential for chromosome end protection. RPA-1 from Leishmania sp., as well as RPA-1 from trypanosomatids, a group of early-divergent protozoa, shows some structural differences compared to higher eukaryote RPA-1. Also, RPA-1 from Leishmania sp., similar to TEBPs, may exert telomeric protective functions. Remarkably, different pieces of evidence have pointed out that trypanosomatids may not have OB fold-containing TEBPs. Moreover, recent data indicate that trypanosomatid RPA-1 may be considered a TEBP since it shares with TEBPs conserved functional and structural features. However, it is still unknown whether the RPA-1 protective telomeric role is exclusive to trypanosomatids or is also present in other primitive eukaryotes. Here, we describe a protocol to obtain highly purified and biologically active Leishmania amazonensis recombinant RPA-1, and to perform molecular modeling and molecular dynamics simulations methods which could be probably applied to functional and structural studies of homologous proteins in other primitive eukaryotes. | - |
Descrição: dc.description | Department of Biophysics and Pharmacology Biosciences Institute São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Chemical and Biological Sciences Biosciences Institute São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Biophysics and Pharmacology Biosciences Institute São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Chemical and Biological Sciences Biosciences Institute São Paulo State University (UNESP) | - |
Formato: dc.format | 169-191 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Methods in Molecular Biology | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Leishmania | - |
Palavras-chave: dc.subject | OB-fold | - |
Palavras-chave: dc.subject | RPA | - |
Palavras-chave: dc.subject | TEBP | - |
Palavras-chave: dc.subject | Telomeres | - |
Palavras-chave: dc.subject | Trypanosomatids | - |
Título: dc.title | RPA-1 from Leishmania sp.: Recombinant Protein Expression and Purification, Molecular Modeling, and Molecular Dynamics Simulations Protocols | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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