Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorMaximino, Mateus D. [UNESP]-
Autor(es): dc.creatorSilva, Carla Y. [UNESP]-
Autor(es): dc.creatorCavalcante, Dalita G.S.M. [UNESP]-
Autor(es): dc.creatorMartin, Cibely S. [UNESP]-
Autor(es): dc.creatorJob, Aldo E. [UNESP]-
Autor(es): dc.creatorOliveira, Osvaldo N.-
Autor(es): dc.creatorAléssio, Priscila [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:46:28Z-
Data de disponibilização: dc.date.available2022-02-22T00:46:28Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.colsurfb.2021.111762-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206206-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206206-
Descrição: dc.descriptionThe inadequate disposal and the difficulty in its removal from water treatment systems have made the endocrine disruptor bisphenol A (BPA) a significant hazard for humans and animals. The molecular-level mechanisms of BPA action are not known in detail, which calls for systematic investigations using cell membrane models. This paper shows that BPA affects Langmuir monolayers and giant unilamellar vesicles (GUVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) used as membrane models, in a concentration-dependent manner and with effects that depend on BPA aggregation. BPA increases DPPC monolayer fluidity in surface pressure isotherms upon interacting with the headgroups through hydrogen bonding, according to polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). In DPPC GUVs, BPA induced wrinkling and distortion in the spherical shape of the vesicles, but this was only observed for fresh solutions where it is not aggregated. BPA also decreased the viability of hamster ovary cells (CHO) in in vitro experiments. In contrast, aged, aggregated BPA solutions did not affect the GUVs and even increased CHO viability. These results may be rationalized in terms of size-dependent effects of BPA, which may be relevant for its endocrine-disrupting effects.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Technology and Applied Sciences-
Descrição: dc.descriptionSão Carlos Institute of Physics University of São Paulo, CP 369-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Technology and Applied Sciences-
Descrição: dc.descriptionCNPq: 304836/2018-4-
Descrição: dc.descriptionCNPq: 422163/2018-0-
Idioma: dc.languageen-
Relação: dc.relationColloids and Surfaces B: Biointerfaces-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBisphenol A-
Palavras-chave: dc.subjectCell membrane models-
Palavras-chave: dc.subjectCell viability-
Palavras-chave: dc.subjectEndocrine disruptors-
Palavras-chave: dc.subjectLangmuir monolayers-
Palavras-chave: dc.subjectVesicles-
Título: dc.titleConsequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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