In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins

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Autor(es): dc.contributorUniversity of Copenhagen-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorMasaryk University-
Autor(es): dc.creatorSrimahaeak, Thanyaporn-
Autor(es): dc.creatorBianchi, Fernanda [UNESP]-
Autor(es): dc.creatorChlumsky, Ondrej-
Autor(es): dc.creatorLarsen, Nadja-
Autor(es): dc.creatorJespersen, Lene-
Data de aceite: dc.date.accessioned2022-02-22T00:46:02Z-
Data de disponibilização: dc.date.available2022-02-22T00:46:02Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jff.2021.104395-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/206052-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/206052-
Descrição: dc.descriptionThis study investigated the effect of four citrus pectins on adhesion of the probiotic Limosilactobacillus fermentum PCC and integrity of the intestinal epithelial monolayers using the Caco-2 cell model. Adhesion of L. fermentum PCC was enhanced (from 35% to 54–73%) in the presence of pectins with high and moderate degree of esterification (DE), whereas it was reduced (to 2%) by a pectin with low DE. Transepithelial electrical resistance (TEER) was significantly increased upon exposure to L. fermentum PCC alone (by 33%) and, to a greater extent, by combinations with pectins (by 44 – 48%), suggesting synergistic action of bacteria and pectins. Increases in TEER were related to transcriptional responses of the tight junction genes, encoding claudin-4 and claudin-2 proteins. The overall results indicated that pectins, when applied in synbiotic combination with L. fermentum PCC, have potential to improve bacterial adhesion and the intestinal epithelial barrier.-
Descrição: dc.descriptionDepartment of Food Science Faculty of Science University of Copenhagen-
Descrição: dc.descriptionDepartment of Food Science UNESP - São Paulo State University-
Descrição: dc.descriptionDepartment of Experimental Biology Masaryk University-
Descrição: dc.descriptionDepartment of Food Science UNESP - São Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationJournal of Functional Foods-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAdhesion-
Palavras-chave: dc.subjectCaco-2 cells-
Palavras-chave: dc.subjectLimosiLactobacillus fermentum PCC-
Palavras-chave: dc.subjectPectins-
Palavras-chave: dc.subjectTight junction-
Título: dc.titleIn-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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