Selection and validation of reference genes by RT-qPCR under photoperiodic induction of flowering in sugarcane (Saccharum spp.)

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorCentro de Cana-
Autor(es): dc.contributorUniversity of Warwick-
Autor(es): dc.creatorda Silva Santos, Paulo H. [UNESP]-
Autor(es): dc.creatorManechini, João R. Vieira-
Autor(es): dc.creatorBrito, Michael S.-
Autor(es): dc.creatorRomanel, Elisson-
Autor(es): dc.creatorVicentini, Renato-
Autor(es): dc.creatorScarpari, Maximiliano-
Autor(es): dc.creatorJackson, Stephen-
Autor(es): dc.creatorPinto, Luciana R.-
Data de aceite: dc.date.accessioned2022-02-22T00:45:50Z-
Data de disponibilização: dc.date.available2022-02-22T00:45:50Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-021-83918-2-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205977-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205977-
Descrição: dc.descriptionAlthough reference genes have previously been used in the expression analysis of genes involved in sugarcane flowering they had not been experimentally validated for stability and consistency of expression between different samples over a wide range of experimental conditions. Here we report the analysis of candidate reference genes in different tissue types, at different temporal time-points, in both short and long day photoperiodic treatments. The stability of the candidate reference genes in all conditions was evaluated with NormFinder, BestKeeper, and RefFinder algorithms that complement each other for a more robust analysis. As the Normfinder algorithm was more appropriate for our experimental conditions, greater emphasis was placed on Normfinder when choosing the most stable genes. UBQ1 and TUB were shown to be the most stable reference genes to use for normalizing RT-qPCR gene expression data during floral induction, whilst 25SrRNA1 and GAPDH were the least stable. Their use as a reference gene pair was validated by analyzing the expression of two differentially expressed target genes (PIL5 and LHP1). The UBQ1/TUB reference genes combination was able to reveal small significant differences in gene expression of the two target genes that were not detectable when using the least stable reference gene combination. These results can be used to inform the choice of reference genes to use in the study of the sugarcane floral induction pathway. Our work also demonstrates that both PIL5 and LHP1 are significantly up-regulated in the initial stages of photoperiodic induction of flowering in sugarcane.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionBiotechnology and Biological Sciences Research Council-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFaculdade de Ciências Agrárias e Veterinárias UNESP, Campus Jaboticabal-
Descrição: dc.descriptionUniversidade Estadual de Campinas UNICAMP-
Descrição: dc.descriptionInstituto de Ciência e Tecnologia da Universidade Federal de São Paulo-
Descrição: dc.descriptionDepartamento de Biotecnologia Escola de Engenharia de Lorena/Universidade de São Paulo EEL/USP-
Descrição: dc.descriptionInstituto Agronômico de Campinas Centro de Cana-
Descrição: dc.descriptionSchool of Life Sciences University of Warwick, Gibbet Hill-
Descrição: dc.descriptionFaculdade de Ciências Agrárias e Veterinárias UNESP, Campus Jaboticabal-
Descrição: dc.descriptionFAPESP: 2016/02679-9-
Descrição: dc.descriptionBiotechnology and Biological Sciences Research Council: 2018/08380-0-
Descrição: dc.descriptionCAPES: Finance Code 001-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleSelection and validation of reference genes by RT-qPCR under photoperiodic induction of flowering in sugarcane (Saccharum spp.)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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