ZIF-8 Metal-Organic Framework Electrochemical Biosensor for the Detection of Protein-Protein Interaction

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorCentro Nacional de Pesquisa em Energia e Materiais (CNPEM)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorTrino, Luciana D.-
Autor(es): dc.creatorAlbano, Luiz G. S.-
Autor(es): dc.creatorGranato, Daniela C.-
Autor(es): dc.creatorSantana, Aline G.-
Autor(es): dc.creatorDe Camargo, Davi H. S.-
Autor(es): dc.creatorCorrea, Catia C.-
Autor(es): dc.creatorBof Bufon, Carlos C. [UNESP]-
Autor(es): dc.creatorPaes Leme, Adriana F.-
Data de aceite: dc.date.accessioned2022-02-22T00:45:46Z-
Data de disponibilização: dc.date.available2022-02-22T00:45:46Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-02-23-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1021/acs.chemmater.0c04201-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205955-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205955-
Descrição: dc.descriptionIn this study, a novel label-free electrochemical biosensor based on the zeolitic imidazole framework (ZIF-8) was developed for monitoring protein-protein interactions (PPIs). ZIF-8 was deposited on interdigitated electrodes and employed as a transducing material and simultaneously carried the thioredoxin-1 (Trx-1) protein, followed by the deposition of increased concentrations of the cytoplasmic domain of a disintegrin and metalloproteinase 17 (ADAM17cyto) known as the Trx-1 binding partner. Structural and morphological characterizations were used to validate and verify the formation of ZIF-8. The ZIF-8 crystals showed a rhombic dodecahedral structure with mainly exposed (011) facets, a mean particle size of 205 (±22) nm, and a ZIF-8 film thickness around 61 (±6) nm. The interaction between Trx-1 and ADAM17cyto proteins was analyzed through electrochemical impedance spectroscopy (EIS). The results indicate a linear and inverse relationship between the impedance responses at 0.1 Hz for ADAM17cyto concentrations from 50 nM to 8 μM, with a coefficient of variation from 1.0% to 11.4%. The proposed biosensor also displayed a significant selectivity and stability verified by using ADAM17cyto mutant and BSA as controls. As a proof-of-concept, we compared the results with a widely used type of PPI assay based on antibody recognition, the solid-phase binding assay, using the same proteins. The solid-phase binding assay was able to detect a significant binding only in ADAM17cyto concentrations above 0.5 μM, with a coefficient of variation varying from 5.4% to 27.5%. The results demonstrate that the developed biosensor was 10× more sensitive and reproducible than the conventional solid-phase binding assay. Furthermore, the developed electrochemical biosensor based on ZIF-8 provides a faster, label-free, and low-cost detection analysis, representing a novel strategy in detecting PPIs.-
Descrição: dc.descriptionLaboratório Nacional de Biociências (LNBio) Centro Nacional de Pesquisa em Energia e Materiais (CNPEM)-
Descrição: dc.descriptionLaboratório Nacional de Nanotecnologia (LNNano) Centro Nacional de Pesquisa em Energia e Materiais (CNPEM)-
Descrição: dc.descriptionDepartamento de Biologia Molecular e Funcional Instituto de Biologia (IB) Universidade de Campinas (UNICAMP)-
Descrição: dc.descriptionDepartamento de Físico-Química Instituto de Química (IQ) Universidade de Campinas (UNICAMP)-
Descrição: dc.descriptionPrograma de Pós Graduação em Ciência e Tecnologia de Materiais (POSMAT) Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionPrograma de Pós Graduação em Ciência e Tecnologia de Materiais (POSMAT) Universidade Estadual Paulista (UNESP)-
Formato: dc.format1293-1306-
Idioma: dc.languageen-
Relação: dc.relationChemistry of Materials-
???dc.source???: dc.sourceScopus-
Título: dc.titleZIF-8 Metal-Organic Framework Electrochemical Biosensor for the Detection of Protein-Protein Interaction-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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