Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | University of California-Los Angeles | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Terasaki Institute for Biomedical Innovation | - |
Autor(es): dc.contributor | The University of Hong Kong | - |
Autor(es): dc.contributor | Kangwon National University | - |
Autor(es): dc.contributor | Molecular Bioeffects Branch (USAFRL/711HPW/RHDJ) | - |
Autor(es): dc.contributor | Michigan State University | - |
Autor(es): dc.contributor | University of California | - |
Autor(es): dc.creator | Barros, Natan R [UNESP] | - |
Autor(es): dc.creator | Kim, Han-Jun | - |
Autor(es): dc.creator | Gouidie, Marcus J | - |
Autor(es): dc.creator | Lee, KangJu | - |
Autor(es): dc.creator | Bandaru, Praveen | - |
Autor(es): dc.creator | Banton, Ethan A | - |
Autor(es): dc.creator | Sarikhani, Einollah | - |
Autor(es): dc.creator | Sun, Wujin | - |
Autor(es): dc.creator | Zhang, Shiming | - |
Autor(es): dc.creator | Cho, Hyun-Jong | - |
Autor(es): dc.creator | Hartel, Martin C | - |
Autor(es): dc.creator | Ostrovidov, Serge | - |
Autor(es): dc.creator | Ahadian, Samad | - |
Autor(es): dc.creator | Hussain, Saber M | - |
Autor(es): dc.creator | Ashammakhi, Nureddin | - |
Autor(es): dc.creator | Dokmeci, Mehmet R | - |
Autor(es): dc.creator | Herculano, Rondinelli D [UNESP] | - |
Autor(es): dc.creator | Lee, Junmin | - |
Autor(es): dc.creator | Khademhosseini, Ali | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:45:29Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:45:29Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-07-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1088/1758-5090/aba503 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/205855 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/205855 | - |
Descrição: dc.description | The skin serves a substantial number of physiological purposes and is exposed to numerous biological and chemical agents owing to its large surface area and accessibility. Yet, current skin models are limited in emulating the multifaceted functions of skin tissues due to a lack of effort on the optimization of biomaterials and techniques at different skin layers for building skin frameworks. Here, we use biomaterial-based approaches and bioengineered techniques to develop a 3D skin model with layers of endothelial cell networks, dermal fibroblasts, and multilayered keratinocytes. Analysis of mechanical properties of gelatin methacryloyl (GelMA)-based bioinks mixed with different portions of alginate revealed bioprinted endothelium could be better modeled to optimize endothelial cell viability with a mixture of 7.5% GelMA and 2% alginate. Matrix stiffness plays a crucial role in modulating produced levels of Pro-Collagen I alpha-1 and matrix metalloproteinase-1 in human dermal fibroblasts and affecting their viability, proliferation, and spreading. Moreover, seeding human keratinocytes with gelatin-coating multiple times proved to be helpful in reducing culture time to create multiple layers of keratinocytes while maintaining their viability. The ability to fabricate selected biomaterials for each layer of skin tissues has implications in the biofabrication of skin systems for regenerative medicine and disease modeling. | - |
Descrição: dc.description | Center for Minimally Invasive Therapeutics (C-MIT) University of California-Los Angeles | - |
Descrição: dc.description | Bioprocess and Biotechnology Department Sãao Paulo State University (Unesp) School of Pharmaceutical Sciences, Km 01 Araraquara-Jau Road | - |
Descrição: dc.description | São Paulo State University (Unesp) Institute of Chemistry, 55 Prof. Francisco Degni Street | - |
Descrição: dc.description | Terasaki Institute for Biomedical Innovation | - |
Descrição: dc.description | Department of Bioengineering Henry Samueli School of Engineering and Applied Sciences University of California-Los Angeles | - |
Descrição: dc.description | Department of Electrical and Electronic Engineering The University of Hong Kong | - |
Descrição: dc.description | College of Pharmacy Kangwon National University | - |
Descrição: dc.description | U.S. Air Force Research Laboratory 711th Human Performance Wing Airman Systems Directorate Bioeffects Division Molecular Bioeffects Branch (USAFRL/711HPW/RHDJ) | - |
Descrição: dc.description | Department of Radiological Sciences University of California-Los Angeles | - |
Descrição: dc.description | Department of Biomedical Engineering Michigan State University | - |
Descrição: dc.description | Department of Chemical and Biomolecular Engineering Henry Samueli School of Engineering and Applied Sciences University of California-Los Angeles | - |
Descrição: dc.description | Jonsson Comprehensive Cancer Centre University of California | - |
Descrição: dc.description | Bioprocess and Biotechnology Department Sãao Paulo State University (Unesp) School of Pharmaceutical Sciences, Km 01 Araraquara-Jau Road | - |
Descrição: dc.description | São Paulo State University (Unesp) Institute of Chemistry, 55 Prof. Francisco Degni Street | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Biofabrication | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | bioprinting | - |
Palavras-chave: dc.subject | dermal fibroblasts,and multilayered keratinocytes | - |
Palavras-chave: dc.subject | gelatin methacryloyl (GelMA) | - |
Palavras-chave: dc.subject | skin tissue engineering | - |
Título: dc.title | Biofabrication of endothelial cell, dermal fibroblast, and multilayered keratinocyte layers for skin tissue engineering | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: