Biofabrication of endothelial cell, dermal fibroblast, and multilayered keratinocyte layers for skin tissue engineering

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of California-Los Angeles-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorTerasaki Institute for Biomedical Innovation-
Autor(es): dc.contributorThe University of Hong Kong-
Autor(es): dc.contributorKangwon National University-
Autor(es): dc.contributorMolecular Bioeffects Branch (USAFRL/711HPW/RHDJ)-
Autor(es): dc.contributorMichigan State University-
Autor(es): dc.contributorUniversity of California-
Autor(es): dc.creatorBarros, Natan R [UNESP]-
Autor(es): dc.creatorKim, Han-Jun-
Autor(es): dc.creatorGouidie, Marcus J-
Autor(es): dc.creatorLee, KangJu-
Autor(es): dc.creatorBandaru, Praveen-
Autor(es): dc.creatorBanton, Ethan A-
Autor(es): dc.creatorSarikhani, Einollah-
Autor(es): dc.creatorSun, Wujin-
Autor(es): dc.creatorZhang, Shiming-
Autor(es): dc.creatorCho, Hyun-Jong-
Autor(es): dc.creatorHartel, Martin C-
Autor(es): dc.creatorOstrovidov, Serge-
Autor(es): dc.creatorAhadian, Samad-
Autor(es): dc.creatorHussain, Saber M-
Autor(es): dc.creatorAshammakhi, Nureddin-
Autor(es): dc.creatorDokmeci, Mehmet R-
Autor(es): dc.creatorHerculano, Rondinelli D [UNESP]-
Autor(es): dc.creatorLee, Junmin-
Autor(es): dc.creatorKhademhosseini, Ali-
Data de aceite: dc.date.accessioned2022-02-22T00:45:29Z-
Data de disponibilização: dc.date.available2022-02-22T00:45:29Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1088/1758-5090/aba503-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205855-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205855-
Descrição: dc.descriptionThe skin serves a substantial number of physiological purposes and is exposed to numerous biological and chemical agents owing to its large surface area and accessibility. Yet, current skin models are limited in emulating the multifaceted functions of skin tissues due to a lack of effort on the optimization of biomaterials and techniques at different skin layers for building skin frameworks. Here, we use biomaterial-based approaches and bioengineered techniques to develop a 3D skin model with layers of endothelial cell networks, dermal fibroblasts, and multilayered keratinocytes. Analysis of mechanical properties of gelatin methacryloyl (GelMA)-based bioinks mixed with different portions of alginate revealed bioprinted endothelium could be better modeled to optimize endothelial cell viability with a mixture of 7.5% GelMA and 2% alginate. Matrix stiffness plays a crucial role in modulating produced levels of Pro-Collagen I alpha-1 and matrix metalloproteinase-1 in human dermal fibroblasts and affecting their viability, proliferation, and spreading. Moreover, seeding human keratinocytes with gelatin-coating multiple times proved to be helpful in reducing culture time to create multiple layers of keratinocytes while maintaining their viability. The ability to fabricate selected biomaterials for each layer of skin tissues has implications in the biofabrication of skin systems for regenerative medicine and disease modeling.-
Descrição: dc.descriptionCenter for Minimally Invasive Therapeutics (C-MIT) University of California-Los Angeles-
Descrição: dc.descriptionBioprocess and Biotechnology Department Sãao Paulo State University (Unesp) School of Pharmaceutical Sciences, Km 01 Araraquara-Jau Road-
Descrição: dc.descriptionSão Paulo State University (Unesp) Institute of Chemistry, 55 Prof. Francisco Degni Street-
Descrição: dc.descriptionTerasaki Institute for Biomedical Innovation-
Descrição: dc.descriptionDepartment of Bioengineering Henry Samueli School of Engineering and Applied Sciences University of California-Los Angeles-
Descrição: dc.descriptionDepartment of Electrical and Electronic Engineering The University of Hong Kong-
Descrição: dc.descriptionCollege of Pharmacy Kangwon National University-
Descrição: dc.descriptionU.S. Air Force Research Laboratory 711th Human Performance Wing Airman Systems Directorate Bioeffects Division Molecular Bioeffects Branch (USAFRL/711HPW/RHDJ)-
Descrição: dc.descriptionDepartment of Radiological Sciences University of California-Los Angeles-
Descrição: dc.descriptionDepartment of Biomedical Engineering Michigan State University-
Descrição: dc.descriptionDepartment of Chemical and Biomolecular Engineering Henry Samueli School of Engineering and Applied Sciences University of California-Los Angeles-
Descrição: dc.descriptionJonsson Comprehensive Cancer Centre University of California-
Descrição: dc.descriptionBioprocess and Biotechnology Department Sãao Paulo State University (Unesp) School of Pharmaceutical Sciences, Km 01 Araraquara-Jau Road-
Descrição: dc.descriptionSão Paulo State University (Unesp) Institute of Chemistry, 55 Prof. Francisco Degni Street-
Idioma: dc.languageen-
Relação: dc.relationBiofabrication-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbioprinting-
Palavras-chave: dc.subjectdermal fibroblasts,and multilayered keratinocytes-
Palavras-chave: dc.subjectgelatin methacryloyl (GelMA)-
Palavras-chave: dc.subjectskin tissue engineering-
Título: dc.titleBiofabrication of endothelial cell, dermal fibroblast, and multilayered keratinocyte layers for skin tissue engineering-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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