Effects of sodium valproate on the chromatin of Triatoma infestans (Klug, 1834) (Hemiptera, Reduviidae) under in vitro culture conditions

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorSuperintendence for Control of Endemic Diseases (SUCEN)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorBassani, Alessandra-
Autor(es): dc.creatorRocha, Marina Amorim-
Autor(es): dc.creatorRodrigues, Vera Lúcia C.C.-
Autor(es): dc.creatorSantos, Douglas S.-
Autor(es): dc.creatorNascimento, Juliana Damieli [UNESP]-
Autor(es): dc.creatorda Rosa, João Aristeu [UNESP]-
Autor(es): dc.creatorMello, Maria Luiza S.-
Data de aceite: dc.date.accessioned2022-02-22T00:45:28Z-
Data de disponibilização: dc.date.available2022-02-22T00:45:28Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.acthis.2021.151695-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205844-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205844-
Descrição: dc.descriptionSodium valproate (VPA) is a classic anticonvulsive, a histone deacetylase inhibitor, and a chromatin remodeling inducer. When injected into specimens of Triatoma infestans, a vector of Chagas disease, VPA affects the chromatin supraorganization of chromocenter heterochromatin in only a few cells of the Malpighian tubules. To test whether this result was explained by the inaccessibility of all of the organ's cells to the drug, we investigated the nuclear phenotypes and global acetylation of lysine 9 in histone H3 (H3K9ac) in Malpighian tubules cultivated in vitro for 1–24 h in the presence of 0.05 mM–1 mM VPA. The present results revealed that the chromatin decondensation event in the chromocenter body, which was detected only under low VPA concentrations up to a 4-h treatment, was not frequent during organ culture, similar to the results for injected insects. Cultivation of T. infestans Malpighian tubules in vitro for 24 h revealed inadequate for cell preservation even in the absence of the drug. Immunofluorescence signals for H3K9ac following VPA treatment showed a slightly increased intensity in the euchromatin, but were never detected in the chromocenter bodies, except with great intensity at their periphery, where the 18S rDNA is located. In conclusion, when VPA affects the chromocenter heterochromatin in this animal cell model, it occurs through a pathway that excludes a classic global H3K9ac mark. Investigation of nonhistone proteins associated with histone methylation marks is still required to further explain the differential response of T. infestans chromatin to VPA.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biology University of Campinas (Unicamp)-
Descrição: dc.descriptionSuperintendence for Control of Endemic Diseases (SUCEN)-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences São Paulo State University (Unesp)-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences São Paulo State University (Unesp)-
Descrição: dc.descriptionCNPq: 304797/2019-7-
Descrição: dc.descriptionCNPq: 307398/2018-8-
Idioma: dc.languageen-
Relação: dc.relationActa Histochemica-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectChromatin remodeling-
Palavras-chave: dc.subjectEpigenetics-
Palavras-chave: dc.subjectHeterochromatin-
Palavras-chave: dc.subjectHistone acetylation-
Palavras-chave: dc.subjectHistone deacetylase inhibitor-
Palavras-chave: dc.subjectInsect-
Título: dc.titleEffects of sodium valproate on the chromatin of Triatoma infestans (Klug, 1834) (Hemiptera, Reduviidae) under in vitro culture conditions-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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