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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Martins, Bruna Rodrigues [UNESP] | - |
Autor(es): dc.creator | Pinto, Thais Silva [UNESP] | - |
Autor(es): dc.creator | da Costa Fernandes, Célio Junior [UNESP] | - |
Autor(es): dc.creator | Bezerra, Fábio [UNESP] | - |
Autor(es): dc.creator | Zambuzzi, Willian Fernando [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:45:14Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:45:14Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2020-12-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1007/s10856-020-06473-8 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/205769 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/205769 | - |
Descrição: dc.description | Although osseointegration and clinical success of titanium (Ti)-implanted materials depend on neovascularization in the reactional peri-implant tissue, very little has been achieved considering the Ti-molecules release on the behavior of endothelial cells. To address this issue, we challenged endothelial cells (HUVECs) with Ti-enriched medium obtained from two types of commercial titanium surfaces [presenting or not dual-acid etching (DAE)] up to 72 h to allow molecular machinery analysis. Our data show that the Ti-enriched medium provokes significant stimulus of angiogenesis-related machinery in endothelial cells by upexpressing VEGFR1, VEGFR2, VEGF, eNOS, and iNOS genes, while the PI3K/Akt signaling pathway was also significantly enhanced. As PI3K/AKT signaling was related to angiogenesis in response to vascular endothelial growth factor (VEGF), we addressed the importance of PI3K/Akt upon Ti-enriched medium responses by concomitantly treating the cells with wortmannin, a well-known PI3K inhibitor. Wortmannin suppressed the angiogenic factors, because VEGF, VEGFR1, and eNOS genes were downregulated in those cells, highlighting the importance of PI3K/AKT signaling on driving angiogenic phenotype and angiogenesis performance within the peri-implant tissue reaction. In conjunction, these data reinforce that titanium-implantable devices modify the metabolism of surrounding cells, such as endothelial cells, probably coupling osteogenesis and angiogenesis processes in peri-implant tissue and then contributing to successfully osseointegration of biomedical titanium-based devices. [Figure not available: see fulltext.]. | - |
Descrição: dc.description | Institute of Biosciences of Botucatu Department of Chemical and Biological Sciences UNESP – São Paulo State University | - |
Descrição: dc.description | Institute of Biosciences of Botucatu Department of Chemical and Biological Sciences UNESP – São Paulo State University | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Journal of Materials Science: Materials in Medicine | - |
???dc.source???: dc.source | Scopus | - |
Título: dc.title | PI3K/AKT signaling drives titanium-induced angiogenic stimulus | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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