PI3K/AKT signaling drives titanium-induced angiogenic stimulus

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorMartins, Bruna Rodrigues [UNESP]-
Autor(es): dc.creatorPinto, Thais Silva [UNESP]-
Autor(es): dc.creatorda Costa Fernandes, Célio Junior [UNESP]-
Autor(es): dc.creatorBezerra, Fábio [UNESP]-
Autor(es): dc.creatorZambuzzi, Willian Fernando [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:45:14Z-
Data de disponibilização: dc.date.available2022-02-22T00:45:14Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10856-020-06473-8-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205769-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205769-
Descrição: dc.descriptionAlthough osseointegration and clinical success of titanium (Ti)-implanted materials depend on neovascularization in the reactional peri-implant tissue, very little has been achieved considering the Ti-molecules release on the behavior of endothelial cells. To address this issue, we challenged endothelial cells (HUVECs) with Ti-enriched medium obtained from two types of commercial titanium surfaces [presenting or not dual-acid etching (DAE)] up to 72 h to allow molecular machinery analysis. Our data show that the Ti-enriched medium provokes significant stimulus of angiogenesis-related machinery in endothelial cells by upexpressing VEGFR1, VEGFR2, VEGF, eNOS, and iNOS genes, while the PI3K/Akt signaling pathway was also significantly enhanced. As PI3K/AKT signaling was related to angiogenesis in response to vascular endothelial growth factor (VEGF), we addressed the importance of PI3K/Akt upon Ti-enriched medium responses by concomitantly treating the cells with wortmannin, a well-known PI3K inhibitor. Wortmannin suppressed the angiogenic factors, because VEGF, VEGFR1, and eNOS genes were downregulated in those cells, highlighting the importance of PI3K/AKT signaling on driving angiogenic phenotype and angiogenesis performance within the peri-implant tissue reaction. In conjunction, these data reinforce that titanium-implantable devices modify the metabolism of surrounding cells, such as endothelial cells, probably coupling osteogenesis and angiogenesis processes in peri-implant tissue and then contributing to successfully osseointegration of biomedical titanium-based devices. [Figure not available: see fulltext.].-
Descrição: dc.descriptionInstitute of Biosciences of Botucatu Department of Chemical and Biological Sciences UNESP – São Paulo State University-
Descrição: dc.descriptionInstitute of Biosciences of Botucatu Department of Chemical and Biological Sciences UNESP – São Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationJournal of Materials Science: Materials in Medicine-
???dc.source???: dc.sourceScopus-
Título: dc.titlePI3K/AKT signaling drives titanium-induced angiogenic stimulus-
Tipo de arquivo: dc.typelivro digital-
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