Integrative platform for the selective recovery of intracellular carotenoids and lipids from: Rhodotorula glutinis CCT-2186 yeast using mixtures of bio-based solvents

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Aveiro-
Autor(es): dc.contributorUniversitas Airlangga Jalan-
Autor(es): dc.contributorCIEPQPF-
Autor(es): dc.creatorMussagy, Cassamo U. [UNESP]-
Autor(es): dc.creatorSantos-Ebinuma, Valéria C. [UNESP]-
Autor(es): dc.creatorKurnia, Kiki A.-
Autor(es): dc.creatorDias, Ana C. R. V.-
Autor(es): dc.creatorCarvalho, Pedro-
Autor(es): dc.creatorCoutinho, Joaõ A. P.-
Autor(es): dc.creatorPereira, Jorge F. B. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:44:51Z-
Data de disponibilização: dc.date.available2022-02-22T00:44:51Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-06-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1039/d0gc02992k-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205631-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205631-
Descrição: dc.descriptionNatural bioactive compounds have been attracting growing interest from the industries as a greeneralternative to synthetic raw materials/products. Rhodotorula glutinis yeast naturally synthesizes added value compounds such as lipids and carotenoids, commonly used for cosmetic, pharmaceutical, and food applications. R. glutinis constitutes a rigid cell-wall structure, requiring energy-saving and efficient cell disruption methods for a sustainable recovery of the intracellular compounds. A simple and ecofriendly technology using mixed bio-based solvents (biosolvents) was evaluated here as an alternative platform to permeabilize yeast cells and to improve the selective recovery of β-carotene, torularhodin, torulene and lipids. The extraction ability of pure and solvent mixtures (methanol, ethanol, ethyl acetate, isopropanol, cyclohexane and 2-methyl tetrahydrofuran) was initially screened, demonstrating the clear impact of using mixtures to improve the extraction yields (up to three-fold increase). After identifying ethyl acetate/ethanol/water as the solvent mixture with a greater capacity to extract carotenoids and lipids, the selective recovery of carotenoids and lipids was enhanced by optimizing the solvent mixture composition ratio. Envisioning the industrial application, an integrated biosolvent-based downstream platform was designed. Two different strategies were investigated to further isolate carotenoids and lipids from R. glutinis biomass and to recycle the ethyl acetate/ethanol/water mixture: (i) precipitation using cold acetone; (ii) sequential liquid-liquid extraction. The integrated process for each strategy was compared with a conventional extraction procedure in terms of recovery efficiencies and its environmental impact. Regardless of the strategy, it is shown that the mixture of ethyl acetate, ethanol and water (15/27/58% w/w) can be reused up to three consecutive extractive cycles, ensuring high extraction efficiency yields, while decreasing the process carbon footprint by about 75% compared to the conventional method.-
Descrição: dc.descriptionDepartment of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences Saõ Paulo State University (UNESP)-
Descrição: dc.descriptionCICECO Aveiro Institute of Materials Department of Chemistry University of Aveiro-
Descrição: dc.descriptionDepartment of Marine Faculty of Fisheries and Marine Universitas Airlangga Jalan, Mulyorejo Kampus C-
Descrição: dc.descriptionCESAM Centre for Environmental and Marine Studies Department of Environment and Planning University of Aveiro-
Descrição: dc.descriptionUniv Coimbra CIEPQPF Department of Chemical Engineering, Rua Sílvio Lima Pólo II-Pinhal de Marrocos-
Descrição: dc.descriptionDepartment of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences Saõ Paulo State University (UNESP)-
Formato: dc.format8478-8494-
Idioma: dc.languageen-
Relação: dc.relationGreen Chemistry-
???dc.source???: dc.sourceScopus-
Título: dc.titleIntegrative platform for the selective recovery of intracellular carotenoids and lipids from: Rhodotorula glutinis CCT-2186 yeast using mixtures of bio-based solvents-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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