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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | University of Aveiro | - |
Autor(es): dc.contributor | Universitas Airlangga Jalan | - |
Autor(es): dc.contributor | CIEPQPF | - |
Autor(es): dc.creator | Mussagy, Cassamo U. [UNESP] | - |
Autor(es): dc.creator | Santos-Ebinuma, Valéria C. [UNESP] | - |
Autor(es): dc.creator | Kurnia, Kiki A. | - |
Autor(es): dc.creator | Dias, Ana C. R. V. | - |
Autor(es): dc.creator | Carvalho, Pedro | - |
Autor(es): dc.creator | Coutinho, Joaõ A. P. | - |
Autor(es): dc.creator | Pereira, Jorge F. B. [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:44:51Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:44:51Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2020-12-06 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1039/d0gc02992k | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/205631 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/205631 | - |
Descrição: dc.description | Natural bioactive compounds have been attracting growing interest from the industries as a greeneralternative to synthetic raw materials/products. Rhodotorula glutinis yeast naturally synthesizes added value compounds such as lipids and carotenoids, commonly used for cosmetic, pharmaceutical, and food applications. R. glutinis constitutes a rigid cell-wall structure, requiring energy-saving and efficient cell disruption methods for a sustainable recovery of the intracellular compounds. A simple and ecofriendly technology using mixed bio-based solvents (biosolvents) was evaluated here as an alternative platform to permeabilize yeast cells and to improve the selective recovery of β-carotene, torularhodin, torulene and lipids. The extraction ability of pure and solvent mixtures (methanol, ethanol, ethyl acetate, isopropanol, cyclohexane and 2-methyl tetrahydrofuran) was initially screened, demonstrating the clear impact of using mixtures to improve the extraction yields (up to three-fold increase). After identifying ethyl acetate/ethanol/water as the solvent mixture with a greater capacity to extract carotenoids and lipids, the selective recovery of carotenoids and lipids was enhanced by optimizing the solvent mixture composition ratio. Envisioning the industrial application, an integrated biosolvent-based downstream platform was designed. Two different strategies were investigated to further isolate carotenoids and lipids from R. glutinis biomass and to recycle the ethyl acetate/ethanol/water mixture: (i) precipitation using cold acetone; (ii) sequential liquid-liquid extraction. The integrated process for each strategy was compared with a conventional extraction procedure in terms of recovery efficiencies and its environmental impact. Regardless of the strategy, it is shown that the mixture of ethyl acetate, ethanol and water (15/27/58% w/w) can be reused up to three consecutive extractive cycles, ensuring high extraction efficiency yields, while decreasing the process carbon footprint by about 75% compared to the conventional method. | - |
Descrição: dc.description | Department of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences Saõ Paulo State University (UNESP) | - |
Descrição: dc.description | CICECO Aveiro Institute of Materials Department of Chemistry University of Aveiro | - |
Descrição: dc.description | Department of Marine Faculty of Fisheries and Marine Universitas Airlangga Jalan, Mulyorejo Kampus C | - |
Descrição: dc.description | CESAM Centre for Environmental and Marine Studies Department of Environment and Planning University of Aveiro | - |
Descrição: dc.description | Univ Coimbra CIEPQPF Department of Chemical Engineering, Rua Sílvio Lima Pólo II-Pinhal de Marrocos | - |
Descrição: dc.description | Department of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences Saõ Paulo State University (UNESP) | - |
Formato: dc.format | 8478-8494 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Green Chemistry | - |
???dc.source???: dc.source | Scopus | - |
Título: dc.title | Integrative platform for the selective recovery of intracellular carotenoids and lipids from: Rhodotorula glutinis CCT-2186 yeast using mixtures of bio-based solvents | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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