Pregnancy decreases O-GlcNAc-modified proteins in systemic arteries of normotensive and spontaneously hypertensive rats

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.creatorTroiano, Jéssica A. [UNESP]-
Autor(es): dc.creatorPotje, Simone R.-
Autor(es): dc.creatorGraton, Murilo E. [UNESP]-
Autor(es): dc.creatorSilva, Daniela S. [UNESP]-
Autor(es): dc.creatorda Costa, Rafael M.-
Autor(es): dc.creatorTostes, Rita C.-
Autor(es): dc.creatorAntoniali, Cristina [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:44:46Z-
Data de disponibilização: dc.date.available2022-02-22T00:44:46Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2020.118885-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205596-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205596-
Descrição: dc.descriptionAim: We determined the role played by O-linked N-acetylglucosamine (O-GlcNAc) of proteins in systemic arteries during late pregnancy in normotensive and hypertensive rats. Main methods: O-GlcNAc levels and O-GlcNAc modification of endothelial nitric oxide synthase (eNOS) were determined in aorta (conductance vessel) and mesenteric arteries (resistance vessels) of non-pregnant (NP) and pregnant (P) Wistar rats and spontaneously hypertensive rats (SHR). Vascular O-GlcNAc-modified proteins, O-GlcNAcase (OGA) and O-GlcNAc transferase (OGT) expression, and OGA activity were analyzed. Concentration–response to phenylephrine (PE) curves were constructed for arteries with and without endothelium. Arteries were treated with vehicle or PugNAc (OGA inhibitor, 100 μmol/L) in the presence of L-NAME (NOS inhibitor, 100 μmol/L). Key findings: The content of vascular O-GlcNAc-modified proteins was lower, OGT and OGA expression did not change, and OGA activity was higher in arteries of P-Wistar rats and P-SHR compared to arteries of NP-groups. Reactivity to PE increased in arteries of P-Wistar rats treated with PugNAc compared to vehicle. O-GlcNAcylation of eNOS decreased in P-SHR compared to NP-SHR. PugNAc partially inhibited the effects of endothelium removal and L-NAME on reactivity to PE in arteries of P-Wistar rats. However, PugNAc did not alter reactivity to PE in arteries of P-SHR. Our data showed that pregnancy decreased the content of vascular O-GlcNAc-modified proteins. Significance: Increased OGA activity and decreased O-GlcNAc modification of eNOS boosts eNOS activity in arteries of P-Wistar rats. In P-SHR, altered OGA activity may lower the content of O-GlcNAc-modified proteins, but decreased OGT activity seems a potential mechanism to reduce glycosylation.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionPrograma de Pós-graduação Multicêntrico em Ciências Fisiológicas SBFis São Paulo State University (Unesp)-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Dentistry Department of Basic Sciences-
Descrição: dc.descriptionUniversity of São Paulo (USP) Faculty of Pharmaceutical Sciences of Ribeirão Preto Department of Physics and Chemistry-
Descrição: dc.descriptionFederal University of Goiás Special Academic Unit of Health Sciences-
Descrição: dc.descriptionUniversity of São Paulo (USP) Ribeirão Preto Medical School Department of Pharmacology-
Descrição: dc.descriptionPrograma de Pós-graduação Multicêntrico em Ciências Fisiológicas SBFis São Paulo State University (Unesp)-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Dentistry Department of Basic Sciences-
Descrição: dc.descriptionFAPESP: 2015/09373-0-
Descrição: dc.descriptionFAPESP: 2016/22180--
Descrição: dc.descriptionCNPq: 302526/2016-1-
Idioma: dc.languageen-
Relação: dc.relationLife Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectArtery-
Palavras-chave: dc.subjecteNOS-
Palavras-chave: dc.subjectHypertension-
Palavras-chave: dc.subjectO-GlcNAc-
Palavras-chave: dc.subjectPregnancy-
Título: dc.titlePregnancy decreases O-GlcNAc-modified proteins in systemic arteries of normotensive and spontaneously hypertensive rats-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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