Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal da Bahia (UFBA)-
Autor(es): dc.creatorQueiroz, Marcela Borsatto [UNESP]-
Autor(es): dc.creatorTorres, Fernanda Ferrari Esteves [UNESP]-
Autor(es): dc.creatorRodrigues, Elisandra Marcia [UNESP]-
Autor(es): dc.creatorViola, Kennia Scapin [UNESP]-
Autor(es): dc.creatorBosso-Martelo, Roberta-
Autor(es): dc.creatorChavez-Andrade, Gisselle Moraima [UNESP]-
Autor(es): dc.creatorGuerreiro-Tanomaru, Juliane Maria [UNESP]-
Autor(es): dc.creatorTanomaru-Filho, Mario [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:44:46Z-
Data de disponibilização: dc.date.available2022-02-22T00:44:46Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.dental.2020.11.014-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205591-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205591-
Descrição: dc.descriptionObjective: To evaluate tricalcium silicate-based (TCS) experimental materials, associated with zirconium oxide (ZrO2), calcium tungstate (CaWO4) or niobium oxide (Nb2O5) radiopacifiers, in comparison with MTA Repair HP (Angelus). Methods: Physicochemical tests: setting time, radiopacity, pH and solubility. In vitro assays: cytotoxicity: MTT and Neutral Red – NR; cell bioactivity: alkaline phosphatase activity (ALP), Alzarin red staining (ARS) and real time PCR (qPCR). Antibacterial activity: direct contact on Enterococcus faecalis in the planktonic form. Physicochemical and ARS data were submitted to ANOVA/Tukey tests; antibacterial activity, to Kruskall–Wallis and Dunn tests; MTT, NR, ALP and qPCR were analyzed by ANOVA/Bonferroni tests (α = 0.05). Results: TCS + CaWO4 presented the longest setting time and MTA HP the shortest. Except for TCS, all the materials presented radiopacity above 3 mmAl. The cements had alkaline pH, antibacterial activity, low solubility and no cytotoxic effects. The highest ALP activity occurred in 14 days, especially to TCS, TCS + ZrO2 and TCS + CaWO4. TCS + ZrO2, TCS + Nb2O5 and MTAHP had higher mineralized nodule formation than those of the negative control (NC). After 7 days, there was no difference in mRNA expression for ALP, when compared to NC. However, after 14 days there was no overexpressed ALP mRNA, especially TCS + Nb2O5, in relation to the CN. All the materials presented antimicrobial action. Significance: The pure tricalcium silicate associated with ZrO2, CaWO4 or Nb2O5 had appropriate physicochemical properties, antibacterial activity, cytocompatibility and induced mineralization in Saos-2, indicating their use as reparative materials.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Restorative Dentistry São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionDepartment of Dentistry Bahia Federal University (UFBA) School of Dentistry-
Descrição: dc.descriptionDepartment of Restorative Dentistry São Paulo State University (UNESP) School of Dentistry-
Formato: dc.format311-320-
Idioma: dc.languageen-
Relação: dc.relationDental Materials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiological properties-
Palavras-chave: dc.subjectCalcium silicate-
Palavras-chave: dc.subjectChemical properties-
Palavras-chave: dc.subjectDental materials-
Palavras-chave: dc.subjectEndodontics-
Palavras-chave: dc.subjectPhysical properties-
Título: dc.titlePhysicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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