Microbial lipase: a new approach for a heterogeneous biocatalyst

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorInstitute of Catalysis (ICP-CSIC)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorTacin, Mariana Vendrasco [UNESP]-
Autor(es): dc.creatorCosta-Silva, Tales A.-
Autor(es): dc.creatorde Paula, Ariela Veloso [UNESP]-
Autor(es): dc.creatorPalomo, Jose M.-
Autor(es): dc.creatorSantos-Ebinuma, Valéria de Carvalho [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:44:44Z-
Data de disponibilização: dc.date.available2022-02-22T00:44:44Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/10826068.2020.1855442-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205580-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205580-
Descrição: dc.descriptionLipases are enzymes employed in several industrial process and their applicability can be increased if these biocatalysts are in the immobilize form. The objective of this work was to study the immobilization of lipase produced by submerged cultivation of Aspergillus sp. by hydrophobic interaction, evaluating its stability and reuse capacity. The immobilization process on octyl-sepharose (C8) and octadecyl-sepabeads (C18) carriers was possible after the removal of oil excess presented in the fermented broth. The results showed that the enzyme was isolated and concentrated in octyl-sepharose with 22% of the initial activity. To increase the amount of enzyme adsorbed on the carrier, 4 immobilization cycles were performed in a row, on the same carrier, with a final immobilization yield of 151.32% and an increase in the specific activity of 136%. The activity test with immobilized lipase showed that the immobilized enzyme maintained 75% of the initial activity after 20 cycles.-
Descrição: dc.descriptionDepartment of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biocatalysis Institute of Catalysis (ICP-CSIC)-
Descrição: dc.descriptionDepartment of Pharmacy School of Pharmaceutical Sciences University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationPreparative Biochemistry and Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAdsorption-
Palavras-chave: dc.subjecthydrophobic carrier-
Palavras-chave: dc.subjectimmobilization-
Palavras-chave: dc.subjectlipases-
Título: dc.titleMicrobial lipase: a new approach for a heterogeneous biocatalyst-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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