Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Institute of Catalysis (ICP-CSIC) | - |
Autor(es): dc.contributor | Universidade de São Paulo (USP) | - |
Autor(es): dc.creator | Tacin, Mariana Vendrasco [UNESP] | - |
Autor(es): dc.creator | Costa-Silva, Tales A. | - |
Autor(es): dc.creator | de Paula, Ariela Veloso [UNESP] | - |
Autor(es): dc.creator | Palomo, Jose M. | - |
Autor(es): dc.creator | Santos-Ebinuma, Valéria de Carvalho [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:44:44Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:44:44Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2019-12-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1080/10826068.2020.1855442 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/205580 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/205580 | - |
Descrição: dc.description | Lipases are enzymes employed in several industrial process and their applicability can be increased if these biocatalysts are in the immobilize form. The objective of this work was to study the immobilization of lipase produced by submerged cultivation of Aspergillus sp. by hydrophobic interaction, evaluating its stability and reuse capacity. The immobilization process on octyl-sepharose (C8) and octadecyl-sepabeads (C18) carriers was possible after the removal of oil excess presented in the fermented broth. The results showed that the enzyme was isolated and concentrated in octyl-sepharose with 22% of the initial activity. To increase the amount of enzyme adsorbed on the carrier, 4 immobilization cycles were performed in a row, on the same carrier, with a final immobilization yield of 151.32% and an increase in the specific activity of 136%. The activity test with immobilized lipase showed that the immobilized enzyme maintained 75% of the initial activity after 20 cycles. | - |
Descrição: dc.description | Department of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Biocatalysis Institute of Catalysis (ICP-CSIC) | - |
Descrição: dc.description | Department of Pharmacy School of Pharmaceutical Sciences University of São Paulo (USP) | - |
Descrição: dc.description | Department of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP) | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Preparative Biochemistry and Biotechnology | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Adsorption | - |
Palavras-chave: dc.subject | hydrophobic carrier | - |
Palavras-chave: dc.subject | immobilization | - |
Palavras-chave: dc.subject | lipases | - |
Título: dc.title | Microbial lipase: a new approach for a heterogeneous biocatalyst | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: