Anti-biofilm effect of ozonized physiological saline solution on peri-implant-related biofilm

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorIndiana University School of Dentistry-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorTonon, Caroline C. [UNESP]-
Autor(es): dc.creatorPanariello, Beatriz H. D.-
Autor(es): dc.creatorSpolidorio, Denise M. P. [UNESP]-
Autor(es): dc.creatorGossweiler, Ana G.-
Autor(es): dc.creatorDuarte, Simone-
Data de aceite: dc.date.accessioned2022-02-22T00:44:44Z-
Data de disponibilização: dc.date.available2022-02-22T00:44:44Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/JPER.20-0333-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205579-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205579-
Descrição: dc.descriptionBackground: Removal of dental plaque and local application of local chemical adjuncts, such as chlorhexidine (CHX), have been used to control and treat peri-implant disease. However, these methods can damage the surface properties of the implants or promote bacterial resistance. The application of ozone as an adjunctive treatment represents a new approach in the management of peri-implantitis. Thus, the purpose of this study was to evaluate the antimicrobial effect of ozonized physiological saline solution in different concentrations against oral biofilms developed on titanium surface. Methods: Single and multi-species biofilms of Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus oralis were formed on titanium specimens for 5 days in anaerobic conditions. Biofilms were treated with ozonized saline solution at different concentrations (25, 50, and 80 μg/NmL), for 30 seconds and 1 minute. CHX (0.12%) and saline solution (0.89% NaCl) were used as positive and negative controls, respectively. Bacterial viability was quantified by Colony Forming Units (CFU mL−1), and biofilm images were acquired by Confocal Laser Scanning Microscopy (CLSM). Data were analyzed by parametric test (ANOVA) with Tukey post-hoc test (P < 0.05). Results: Ozonized saline solution showed antibiofilm activity at a concentration of 80 μg/NmL for 30 seconds and 1 minute, reducing, mainly, Porphyromonas gingivalis viability, with 2.78 and 1.7 log10 CFU mL−1 of reduction in both single and multi-specie biofilms, respectively, when compared to the control (saline), whereas CHX reduced 1.4 and 1.2 log10 CFU mL−1. Conclusion: Ozonized saline solution has antibiofilm activity, with better effect when applied for 1 minute at 80 μg/NmL, being a promising candidate therapy for the treatment of peri-implant diseases.-
Descrição: dc.descriptionDepartment of Cariology Operative Dentistry and Dental Public Health Indiana University School of Dentistry-
Descrição: dc.descriptionDepartment of Oral Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Oral Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationJournal of Periodontology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiofilm-
Palavras-chave: dc.subjectFusobacterium nucleatum-
Palavras-chave: dc.subjectozono therapy-
Palavras-chave: dc.subjectperi-implantitis-
Palavras-chave: dc.subjectPorphyromonas gingivalis-
Palavras-chave: dc.subjectStreptococcus oralis-
Título: dc.titleAnti-biofilm effect of ozonized physiological saline solution on peri-implant-related biofilm-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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