Lesion of Serotonergic Afferents to the Retrotrapezoid Nucleus Impairs the Tachypneic Response to Hypercapnia in Unanesthetized Animals

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.creatorLeirão, Isabela P. [UNESP]-
Autor(es): dc.creatorColombari, Débora S.A. [UNESP]-
Autor(es): dc.creatorda Silva, Glauber S.F.-
Autor(es): dc.creatorZoccal, Daniel B. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:44:41Z-
Data de disponibilização: dc.date.available2022-02-22T00:44:41Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.neuroscience.2020.11.005-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205574-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205574-
Descrição: dc.descriptionHypercapnia promotes an increase in pulmonary ventilation due to the stimulation of brainstem chemosensory cells that are connected to the respiratory network. Among these cells are the raphe serotonergic neurons which widely send projections to distinct central respiratory compartments. Nevertheless, the physiological role of specific raphe serotonergic projections to other chemosensitive sites on the emergence of hypercapnia ventilatory response in vivo still remains to be elucidated. Here we investigated whether the ventilatory response to hypercapnia requires serotonergic inputs to the chemosensitive cells of the retrotrapezoid nucleus (RTN) in the ventrolateral medulla. To test this, pulmonary ventilation was evaluated under baseline conditions and during hypercapnia (7% CO2) in unanesthetized juvenile Holtzman rats (60–90 g) that received bilateral microinjections of either vehicle (control) or anti-SERT-SAP (0.1 mM, 10 pmol/100 nl) toxin in the RTN to retrogradely destroy serotonergic afferents to this region. Fifteen days after microinjections, baseline ventilation was not different between anti-SERT-SAP (n = 8) and control animals (n = 9). In contrast, the ablation of RTN-projecting serotonergic neurons markedly attenuated the hypercapnia-induced increase in respiratory frequency which was correlated with reduced numbers of serotonergic neurons in the raphe obscurus and magnus, but not in the raphe pallidus. The increase in tidal volume during hypercapnia was not significantly affected by anti-SERT-SAP microinjections in the RTN. Our data indicate that serotoninergic neurons that send projections to the RTN region are required for the processing of ventilatory reflex response during exposure to high CO2 in unanesthetized conditions.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Physiology and Biophysics. Institute of Biological Sciences Federal University of Minas Gerais (ICB/UFMG)-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionCNPq: 132363/2018-6-
Descrição: dc.descriptionFAPESP: 2013/17251-6-
Descrição: dc.descriptionFAPESP: 2018/04439-0-
Descrição: dc.descriptionFAPESP: 2018/21000-2-
Descrição: dc.descriptionCNPq: 310331/2017-0-
Descrição: dc.descriptionCNPq: 403769/2016-7-
Descrição: dc.descriptionCNPq: 408950/2018-8-
Formato: dc.format63-77-
Idioma: dc.languageen-
Relação: dc.relationNeuroscience-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcarbon dioxide-
Palavras-chave: dc.subjectcentral chemoreception-
Palavras-chave: dc.subjectmedullary raphe-
Palavras-chave: dc.subjectventilation-
Título: dc.titleLesion of Serotonergic Afferents to the Retrotrapezoid Nucleus Impairs the Tachypneic Response to Hypercapnia in Unanesthetized Animals-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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