Transcriptional profiling of embryo cryotolerance

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of ABC-
Autor(es): dc.contributorFederal University of Pampa-
Autor(es): dc.contributorABS Pecplan-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorMarsico, Thamiris V.-
Autor(es): dc.creatorCaetano, Diana P.-
Autor(es): dc.creatorRodrigues, Rafaela-
Autor(es): dc.creatorValente, Roniele S.-
Autor(es): dc.creatorFontes, Patricia K. [UNESP]-
Autor(es): dc.creatorMesquita, Fernando S.-
Autor(es): dc.creatorAndrade, Sónia C. da Silva-
Autor(es): dc.creatorBasso, Andréa C.-
Autor(es): dc.creatorNogueira, Marcelo F. G. [UNESP]-
Autor(es): dc.creatorSudano, Mateus J.-
Data de aceite: dc.date.accessioned2022-02-22T00:44:40Z-
Data de disponibilização: dc.date.available2022-02-22T00:44:40Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/mrd.23436-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205565-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205565-
Descrição: dc.descriptionThe cryosurvival of embryos is a complex process involving dynamic and integrated morphological, functional, and molecular changes. Here, we evaluated the transcriptional profiling of bovine embryos possessing high and low cryotolerance (HC and LC, respectively) by assessing the resumption of development. Embryos were produced in vitro (N = 1137) and cryopreserved (N = 894). Blastocysts samples possessed pronounced group individualization at RNA sequencing. A total of 114 genes were differentially expressed, and 27 and 84 genes were upregulated in HC and LC, respectively. Among the over-represented biological functions, cellular growth and proliferation, cell death and survival, and organismal survival were predicted to be activated, while cellular movement and cell-to-cell signaling were predicted to be inhibited in HC embryos. Enriched canonical pathways and upstream regulators related to cellular proliferation and survival (HC), inflammatory processes, and cell death (LC) were predicted to represent two embryonic molecular profiles present during the resumption of development after cryopreservation. The marked contrast in transcriptional profiles between HC and LC strongly suggests the influence of embryonic competence after cryopreservation on its respective transcriptome and indicated that HC and LC presented two different molecular strategies to overcome cryopreservation-related stress and resume postcryopreservation development.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCenter for Natural and Human Sciences Federal University of ABC-
Descrição: dc.descriptionSchool of Veterinary Medicine Federal University of Pampa-
Descrição: dc.descriptionIn vitro Brazil – IVB ABS Pecplan-
Descrição: dc.descriptionDepartment of Pharmacology Laboratory of Phytomedicines Pharmacology and Biotechnology Institute of Biosciences University of São Paulo State (Unesp)-
Descrição: dc.descriptionDepartment of Genetics and Evolutionary Biology University of São Paulo-
Descrição: dc.descriptionDepartment of Biological Science School of Sciences and Languages University of São Paulo State-
Descrição: dc.descriptionDepartment of Genetics and Evolution Federal University of São Carlos-
Descrição: dc.descriptionDepartment of Pharmacology Laboratory of Phytomedicines Pharmacology and Biotechnology Institute of Biosciences University of São Paulo State (Unesp)-
Formato: dc.format1245-1259-
Idioma: dc.languageen-
Relação: dc.relationMolecular Reproduction and Development-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbovine-
Palavras-chave: dc.subjectcryosurvival-
Palavras-chave: dc.subjectembryonic competence-
Palavras-chave: dc.subjectpostcryopreservation resumption of development-
Palavras-chave: dc.subjectquality-
Título: dc.titleTranscriptional profiling of embryo cryotolerance-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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