Non-coding RNAs repressive role in post-transcriptional processing of RUNX2 during the acquisition of the osteogenic phenotype of periodontal ligament mesenchymal stem cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Taubaté-
Autor(es): dc.contributorSão Paulo-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorColumbia University Medical Center-
Autor(es): dc.contributorPaulista University-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.creatorAssis, Rahyza I.F.-
Autor(es): dc.creatorFeltran, Geórgia da S. [UNESP]-
Autor(es): dc.creatorSilva, Maria Eduarda Salomão-
Autor(es): dc.creatorPalma, Iasmin Caroline do Rosário-
Autor(es): dc.creatorRovai, Emanuel Silva-
Autor(es): dc.creatorMiranda, Taís Browne de-
Autor(es): dc.creatorFerreira, Marcel Rodrigues [UNESP]-
Autor(es): dc.creatorZambuzzi, Willian F. [UNESP]-
Autor(es): dc.creatorBirbrair, Alexander-
Autor(es): dc.creatorAndia, Denise C.-
Autor(es): dc.creatorda Silva, Rodrigo A.-
Data de aceite: dc.date.accessioned2022-02-22T00:44:34Z-
Data de disponibilização: dc.date.available2022-02-22T00:44:34Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ydbio.2020.10.012-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205528-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205528-
Descrição: dc.descriptionMesenchymal stem cells are candidates for therapeutic strategies in periodontal repair due to their osteogenic potential. In this study, we identified epigenetic markers during osteogenic differentiation, taking advantage of the individual pattern of mesenchymal cells of the periodontal ligament with high (h-PDLCs) and low (l-PDLCs) osteogenic capacity. We found that the involvement of non-coding RNAs in the regulation of the RUNX2 gene is strongly associated with high osteogenic potential. Moreover, we evaluated miRs and genes that encode enzymes to process miRs and their biogenesis. Our data show the high expression of the XPO5 gene, and miRs 7 and 22 observed in the l-PDLCs might be involved in acquiring osteogenic potential, suppressing RUNX2 gene expression. Further, an inversely proportional correlation between lncRNAs (HOTAIR and HOTTIP) and RUNX2 gene expression was observed in both l- and h-PDLCs, and it was also related to the distinct osteogenic phenotypes. Thus, our results indicate the low expression of XPO5 in h-PDLC might be the limiting point for blocking the miRs biogenesis, allowing the high gene expression of RUNX2. In accordance, the low expression of miRs, HOTAIR, and HOTTIP could be a prerequisite for increased osteogenic potential in h-PDLCs. These results will help us to better understand the underlying mechanisms of osteogenesis, considering the heterogeneity in the osteogenic potential of PDLCs that might be related to a distinct transcriptional profile of lncRNAs and the biogenesis machinery.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFaculty of Dentistry University of Taubaté-
Descrição: dc.descriptionProgram in Environmental and Experimental Pathology Paulista University São Paulo-
Descrição: dc.descriptionLab. of Bioassays and Cellular Dynamics Department of Chemical and Biological Sciences Institute of Biosciences UNESP – São Paulo State University, São Paulo-
Descrição: dc.descriptionDepartment of Pathology Federal University of Minas Gerais-
Descrição: dc.descriptionDepartment of Radiology Columbia University Medical Center-
Descrição: dc.descriptionSchool of Dentistry Health Science Institute Paulista University-
Descrição: dc.descriptionDepartment of Prosthodontics and Periodontics Piracicaba Dental School University of Campinas-
Descrição: dc.descriptionLab. of Bioassays and Cellular Dynamics Department of Chemical and Biological Sciences Institute of Biosciences UNESP – São Paulo State University, São Paulo-
Descrição: dc.descriptionCNPq: ODO 154/2019-
Formato: dc.format37-48-
Idioma: dc.languageen-
Relação: dc.relationDevelopmental Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEpigenetic background-
Palavras-chave: dc.subjectOsteogenic potential-
Palavras-chave: dc.subjectPeriodontal regeneration-
Palavras-chave: dc.subjectStem cells-
Palavras-chave: dc.subjectTissue engineering-
Título: dc.titleNon-coding RNAs repressive role in post-transcriptional processing of RUNX2 during the acquisition of the osteogenic phenotype of periodontal ligament mesenchymal stem cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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