Improvement of some chemical and biological methods for the efficient production of xylanases, xylooligosaccharides and lignocellulose from sugar cane bagasse

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorCarvalho, Ana Flavia Azevedo [UNESP]-
Autor(es): dc.creatorFigueiredo, Franciane Cristina de [UNESP]-
Autor(es): dc.creatorCampioni, Tania Sila [UNESP]-
Autor(es): dc.creatorPastore, Glaucia Maria-
Autor(es): dc.creatorde Oliva Neto, Pedro [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:44:34Z-
Data de disponibilização: dc.date.available2022-02-22T00:44:34Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.biombioe.2020.105851-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205524-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205524-
Descrição: dc.descriptionThe use of agroindustrial waste for chemicals, biofuels and food ingredients production has been studied considering limitations of natural resources. Therefore, sugarcane bagasse was studied to produce arabinoxylan, xylanases, xylooligosaccharides and lignocellulose by the improvement of some technologies. A comparison of two methods of extraction of arabinoxylan was performed. Arabinoxylan was obtained in a soluble fraction in a better extraction by KOH method with higher yield (72.1%) and efficiency (84%) than H2O2 method, which only produced 64.9 and 43.3%, respectively. Lignocellulose was obtained after filtration, as the solid fraction, representing 66.2% (KOH) and 78.8% (H2O2 method) of the total biomass. The endoxylanase was produced by Aspergillus fumigatus CCT7732 in submerged fermentation with bagasse culture medium reaching 40.2 U/mL in optimal conditions, pH 5.0 and 31.5 °C. In addition, NH4H2PO4 was efficient as phosphorus and nitrogen sources for xylanases production in the culture, when the more expensive salts K2HPO4 and KH2PO4 were replaced. An enzymatic hydrolysis of arabinoxylan in Xylooligosaccharides (XOS) by xylanases of A. fumigatus resulted in 3.3% (m/v) XOS, 46.9% yield using 7% arabinoxylan, 350 U/g of xylanases at 50 °C in 48 h. These methods provided a prebiotic XOS since mainly xylobiose and xylotriose were produced with potential applications for food and feed. The set of chemical and biological methods applied in bagasse indicated that these methods should be evaluated in scale up of an integrated biosystem including economic studies for industrial purposes.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Food Science School of Food Engineering State University of Campinas (UNICAMP), Rua Monteiro Lobato, 80, ZIP Code-
Descrição: dc.descriptionInstitute for Research in Bioenergy (Associated Laboratory - IPBEN) Bioprocess Unit São Paulo State University (UNESP), Av. Dom Antonio, 2100-
Descrição: dc.descriptionInstitute for Research in Bioenergy (Associated Laboratory - IPBEN) Bioprocess Unit São Paulo State University (UNESP), Av. Dom Antonio, 2100-
Descrição: dc.descriptionCNPq: 151348/2013-8-
Descrição: dc.descriptionFAPESP: 2011/09100-2-
Idioma: dc.languageen-
Relação: dc.relationBiomass and Bioenergy-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAlkaline pretreatment-
Palavras-chave: dc.subjectAspergillus fumigatus-
Palavras-chave: dc.subjectImprovement of enzyme production-
Palavras-chave: dc.subjectLignocellulosic residues-
Palavras-chave: dc.subjectXylobiose-
Título: dc.titleImprovement of some chemical and biological methods for the efficient production of xylanases, xylooligosaccharides and lignocellulose from sugar cane bagasse-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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