A novel mechanism of β-glucosidase stimulation through a monosaccharide binding-induced conformational change

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorBrazilian Center for Research in Energy and Materials (CNPEM)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de Sorocaba (UNISO)-
Autor(es): dc.creatorCorrêa, Thamy L.R.-
Autor(es): dc.creatorFranco Cairo, João Paulo L.-
Autor(es): dc.creatorCota, Junio-
Autor(es): dc.creatorDamasio, André-
Autor(es): dc.creatorOliveira, Leandro C. [UNESP]-
Autor(es): dc.creatorSquina, Fabio M.-
Data de aceite: dc.date.accessioned2022-02-22T00:44:34Z-
Data de disponibilização: dc.date.available2022-02-22T00:44:34Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2020.11.001-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205522-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205522-
Descrição: dc.descriptionIt is urgent the transition from a fossil fuel-based economy to a sustainable bioeconomy based on bioconversion technologies using renewable plant biomass feedstocks to produce high chemicals, bioplastics, and biofuels. β-Glucosidases are key enzymes responsible for degrading the plant cell wall polymers, as they cleave glucan-based oligo- and polysaccharides to generate glucose. Monosaccharide-tolerant or -stimulated β-glucosidases have been reported in the past decade. Here, we describe a novel mechanism of β-glucosidase stimulation by glucose and xylose. The glycoside hydrolase 1 family β-glucosidase from Thermotoga petrophila (TpBgl1) displays a typical glucose stimulation mechanism based on an increased Vmax and decreased Km in response to glucose. Through molecular docking and dynamics analyses, we mapped putative monosaccharide binding regions (BRs) on the surface of TpBgl1. Our results indicate that after interaction with glucose or xylose at BR1 site, an adjacent loop region assumes an extended conformation, which increases the entrance to the TpBgl1 active site, improving product formation. Biochemical assays with TpBgl1 BR1 mutants, TpBgl1D49A/Y410A and TpBgl1D49K/Y410H, resulted in decreasing and abolishing monosaccharide stimulation, respectively. These mutations also impaired the BR1 looping extension responsible for monosaccharide stimulation. This study provides a molecular basis for the rational design of β-glucosidases for biotechnological applications.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionFundação para o Desenvolvimento da UNESP (FUNDUNESP)-
Descrição: dc.descriptionBrazilian Biorenewables National Laboratory (LNBR) Brazilian Center for Research in Energy and Materials (CNPEM)-
Descrição: dc.descriptionDepartment of Biochemistry and Tissue Biology Institute of Biology (IB) University of Campinas (UNICAMP)-
Descrição: dc.descriptionInstituto de Ciências Agrárias (ICA) Universidade Federal de Minas Gerais (UFMG)-
Descrição: dc.descriptionSão Paulo State University (Unesp) Department of Physics Institute of Biosciences Humanities and Exact Sciences-
Descrição: dc.descriptionPrograma de Processos Tecnológicos e Ambientais Universidade de Sorocaba (UNISO)-
Descrição: dc.descriptionSão Paulo State University (Unesp) Department of Physics Institute of Biosciences Humanities and Exact Sciences-
Descrição: dc.descriptionCNPq: 150664/2013-3-
Descrição: dc.descriptionFAPESP: 2010/18198-3-
Descrição: dc.descriptionFAPESP: 2011/13242-7-
Descrição: dc.descriptionFAPESP: 2012/20549-4-
Descrição: dc.descriptionFAPESP: 2015/50590-4-
Descrição: dc.descriptionFAPESP: 2016/09950-0-
Descrição: dc.descriptionFUNDUNESP: 2532/002/14-PROPe/CDC-
Descrição: dc.descriptionCNPq: 304816/2017-5-
Descrição: dc.descriptionCNPq: 305748/2017-3-
Descrição: dc.descriptionCNPq: 428527/2018-3-
Descrição: dc.descriptionCNPq: 442352/2014-0-
Formato: dc.format1188-1196-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Biological Macromolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBioeconomy-
Palavras-chave: dc.subjectGH1-
Palavras-chave: dc.subjectMolecular dynamics simulation-
Palavras-chave: dc.subjectThermotoga petrophila-
Palavras-chave: dc.subjectβ-Glucosidases-
Título: dc.titleA novel mechanism of β-glucosidase stimulation through a monosaccharide binding-induced conformational change-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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