Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorAntioquia University-
Autor(es): dc.creatorBatista, Thiago Barbosa [UNESP]-
Autor(es): dc.creatorFernandez, Geysson Javier-
Autor(es): dc.creatorDa Silva, Tiago Alexandre [UNESP]-
Autor(es): dc.creatorMaia, Júlio [UNESP]-
Autor(es): dc.creatorDa Silva, Edvaldo Aparecido Amaral [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:44:06Z-
Data de disponibilização: dc.date.available2022-02-22T00:44:06Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-08-19-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/aobpla/plaa041-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205352-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205352-
Descrição: dc.descriptionSeed priming is widely used in commercial seeds and its main function is to accelerate and synchronize seed germination. Undesirably, primed seeds show reduced longevity and treatments like heat shock have been shown to improve longevity in primed seeds. Nonetheless, the effect of heat shock treatment on primed seeds at the mRNA level is not known. Thus, the aim of this work was to investigate the effect of heat shock treatment on the longevity of primed tomato (Solanum lycopersicum) seeds at the physiological and transcriptome levels. Tomato seeds were primed and dried (control). Alternatively, primed seeds were subjected to heat shock treatment (38 °C/32 % relative humidity) before drying. Germination, vigor and longevity were evaluated. Transcriptome analysis was performed by RNA sequencing (RNA-seq) from biological samples collected immediately after priming and another samples collected from primed seeds followed by the heat shock treatments. The gene expression was validated by quantitative real time PCR (RT-qPCR). We showed that applying heat shock treatment after priming increased germination speed, enhanced seed longevity and preserved the vigor during storage of primed tomato seeds. Through transcriptome analysis, 368 differentially expressed genes were identified, from which 298 genes were up-regulated and 70 were down-regulated. We showed the increase of mRNA levels of HEAT SHOCK FACTOR-like and HEAT SHOCK PROTEIN-like chaperone genes, suggesting the involvement of the proteins coded by these transcripts in the enhancement of longevity in primed tomato seeds. The heat shock treatment after priming enhances and preserves the vigor of tomato primed seeds during storage. In addition, improves seed longevity through the increase in the expression of transcripts related to protection by response to stress.-
Descrição: dc.descriptionDepartment of Plant Production Sao Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Biology Antioquia University-
Descrição: dc.descriptionDepartment of Plant Production Sao Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationAoB PLANTS-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectChaperone molecules-
Palavras-chave: dc.subjectimproved longevity-
Palavras-chave: dc.subjectprimed seed-
Palavras-chave: dc.subjectseed conservation-
Palavras-chave: dc.subjectseed quality-
Palavras-chave: dc.subjectSolanum lycopersicum L-
Palavras-chave: dc.subjectstorage-
Título: dc.titleTranscriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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