Draft genome sequence of mcr-1-mediated colistin-resistant Escherichia coli ST359 from chicken carcasses in Northeastern Brazil

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Autor(es): dc.contributorUniversidade Federal da Paraíba (UFPB)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorOhio State University (OSU)-
Autor(es): dc.creatorVasconcelos, Priscylla C.-
Autor(es): dc.creatorLeite, Elma L.-
Autor(es): dc.creatorAraújo, Wydemberg J.-
Autor(es): dc.creatorSilva, Núbia M.V-
Autor(es): dc.creatorSaraiva, Mauro M.S. [UNESP]-
Autor(es): dc.creatorSantos Filho, Lauro-
Autor(es): dc.creatorFreitas Neto, Oliveiro C.-
Autor(es): dc.creatorGivisiez, Patricia E.N. [UNESP]-
Autor(es): dc.creatorOliveira, Celso J.B. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:43:54Z-
Data de disponibilização: dc.date.available2022-02-22T00:43:54Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jgar.2020.08.016-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/205274-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/205274-
Descrição: dc.descriptionObjectives: Considering that polymyxin is a drug of last resort in the treatment of humans infected by multidrug-resistant bacteria, the occurrence of plasmid-mediated colistin resistance mcr gene among Gram-negative bacteria in foods must be investigated. We present herein the draft genome sequence of a phenotypically colistin-resistant Escherichia coli carrying mcr-1 in chicken carcasses from a public market. Methods: Total genomic DNA from the strain was sequenced by means of the Illumina MiSeq. The assembled contigs were annotated and manually curated. In silico analyses were performed to detect significant epidemiologic (serotyping and MLST) and structural features related plasmids identification, virulence and resistome. Results: The ST359 E. coli strain presented a conserved 747 bp mcr-1 gene within a 9431 kb contig compatible with the IncX4 plasmid, which has been identified as a key vector for the global dissemination of mcr determinants among Enterobacteriacea. Other genes encoding for multidrug resistance such as blaCTX-M-2 and blaTEM-1B, and the virulence factors astA, cma, gad, iroN, ipfA, mchF were also detected. Conclusion: We reported a draft genome of a colistin-resistant E. coli ST359 associated with an IncX4 plasmid containing the gene mcr-1. The genomic data can be useful in epidemiological and evolutionary investigations on the spread of colistin-resistance among Enterobacteriacea in the food chain.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFinanciadora de Estudos e Projetos-
Descrição: dc.descriptionDepartment of Animal Science College for Agricultural Sciences Federal University of Paraiba (CCA/UFPB)-
Descrição: dc.descriptionDepartment of Veterinary Pathology School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp)-
Descrição: dc.descriptionDepartment of Pharmaceutical Sciences Health Sciences Center Federal University of Paraiba (CCS/UFPB)-
Descrição: dc.descriptionDepartment of Preventive Veterinary Medicine Veterinary School Federal University of Minas Gerais (UFMG)-
Descrição: dc.descriptionGlobal One Health Initiative (GOHi) Ohio State University (OSU)-
Descrição: dc.descriptionDepartment of Veterinary Pathology School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp)-
Formato: dc.format135-136-
Idioma: dc.languageen-
Relação: dc.relationJournal of Global Antimicrobial Resistance-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntimicrobial resistance-
Palavras-chave: dc.subjectChicken-
Palavras-chave: dc.subjectColistin-
Palavras-chave: dc.subjectIncX4-
Palavras-chave: dc.subjectmcr-1-
Título: dc.titleDraft genome sequence of mcr-1-mediated colistin-resistant Escherichia coli ST359 from chicken carcasses in Northeastern Brazil-
Aparece nas coleções:Repositório Institucional - Unesp

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