Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade de São Paulo (USP) | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Freire, Rominne Karla Barros | - |
Autor(es): dc.creator | Mendonça, Carlos Miguel Nóbrega | - |
Autor(es): dc.creator | Ferraro, Rafael Bertelli | - |
Autor(es): dc.creator | Moguel, Ignacio Sánchez | - |
Autor(es): dc.creator | Tonso, Aldo | - |
Autor(es): dc.creator | Lourenço, Felipe Rebello | - |
Autor(es): dc.creator | Santos, João Henrique Picado Madalena | - |
Autor(es): dc.creator | Sette, Lara Durães [UNESP] | - |
Autor(es): dc.creator | Pessoa Junior, Adalberto | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:35:26Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:35:26Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2019-12-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1080/10826068.2020.1815053 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/202103 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/202103 | - |
Descrição: dc.description | L-asparaginase (ASNase) is an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Commercial bacterial ASNases increase patient survival, but the consequent immunological reactions remain a challenge. Yeasts ASNase is closer to human congeners and could lead to lower side effects. Among 134 yeast strains isolated from marine-sediments in King George Island, Antarctica, nine were L-asparaginase producing yeasts and glutaminase-free. Leucosporidium muscorum CRM 1648 yielded the highest ASNase activity (490.41 U.L−1) and volumetric productivity (5.12 U.L−1 h−1). Sucrose, yeast extract and proline were the best carbon and nitrogen sources to support growth and ASNase production. A full factorial design analysis pointed the optimum media condition for yeast growth and ASNase yield: 20 g L−1 sucrose, 15 g L−1 yeast extract and 20 g L−1 proline, which resulted in 4582.5 U L−1 and 63.64 U L−1 h−1 of ASNase and volumetric productivity, respectively. Analysis of temperature, pH, inoculum and addition of seawater indicated the best condition for ASNase production by this yeast: 12–15 °C, pH 5.5–6.5 and seawater >25% (v/v). Inoculum concentration seems not to interfere. This work is pioneer on the production of ASNase by cold-adapted yeasts, highlighting the potential of these microbial resources as a source of glutaminase-free L-asparaginase for commercial purposes. | - |
Descrição: dc.description | Department of Biochemical and Pharmaceutical Technology School of Pharmaceutical Sciences University of Sao Paulo | - |
Descrição: dc.description | Department of Chemical Engineering Polytechnic School University of Sao Paulo | - |
Descrição: dc.description | Department of Pharmacy School of Pharmaceutical Sciences University of Sao Paulo | - |
Descrição: dc.description | Department of General and Applied Biology Institute of Biosciences State University Julio de Mesquita Filho (UNESP) | - |
Descrição: dc.description | Department of General and Applied Biology Institute of Biosciences State University Julio de Mesquita Filho (UNESP) | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Preparative Biochemistry and Biotechnology | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Cold-adapted yeast | - |
Palavras-chave: dc.subject | enzyme production | - |
Palavras-chave: dc.subject | glutaminase-free asparaginase | - |
Palavras-chave: dc.subject | L-asparaginase | - |
Palavras-chave: dc.subject | Leucosporidium muscorum | - |
Título: dc.title | Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: