Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorFreire, Rominne Karla Barros-
Autor(es): dc.creatorMendonça, Carlos Miguel Nóbrega-
Autor(es): dc.creatorFerraro, Rafael Bertelli-
Autor(es): dc.creatorMoguel, Ignacio Sánchez-
Autor(es): dc.creatorTonso, Aldo-
Autor(es): dc.creatorLourenço, Felipe Rebello-
Autor(es): dc.creatorSantos, João Henrique Picado Madalena-
Autor(es): dc.creatorSette, Lara Durães [UNESP]-
Autor(es): dc.creatorPessoa Junior, Adalberto-
Data de aceite: dc.date.accessioned2022-02-22T00:35:26Z-
Data de disponibilização: dc.date.available2022-02-22T00:35:26Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/10826068.2020.1815053-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/202103-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/202103-
Descrição: dc.descriptionL-asparaginase (ASNase) is an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Commercial bacterial ASNases increase patient survival, but the consequent immunological reactions remain a challenge. Yeasts ASNase is closer to human congeners and could lead to lower side effects. Among 134 yeast strains isolated from marine-sediments in King George Island, Antarctica, nine were L-asparaginase producing yeasts and glutaminase-free. Leucosporidium muscorum CRM 1648 yielded the highest ASNase activity (490.41 U.L−1) and volumetric productivity (5.12 U.L−1 h−1). Sucrose, yeast extract and proline were the best carbon and nitrogen sources to support growth and ASNase production. A full factorial design analysis pointed the optimum media condition for yeast growth and ASNase yield: 20 g L−1 sucrose, 15 g L−1 yeast extract and 20 g L−1 proline, which resulted in 4582.5 U L−1 and 63.64 U L−1 h−1 of ASNase and volumetric productivity, respectively. Analysis of temperature, pH, inoculum and addition of seawater indicated the best condition for ASNase production by this yeast: 12–15 °C, pH 5.5–6.5 and seawater >25% (v/v). Inoculum concentration seems not to interfere. This work is pioneer on the production of ASNase by cold-adapted yeasts, highlighting the potential of these microbial resources as a source of glutaminase-free L-asparaginase for commercial purposes.-
Descrição: dc.descriptionDepartment of Biochemical and Pharmaceutical Technology School of Pharmaceutical Sciences University of Sao Paulo-
Descrição: dc.descriptionDepartment of Chemical Engineering Polytechnic School University of Sao Paulo-
Descrição: dc.descriptionDepartment of Pharmacy School of Pharmaceutical Sciences University of Sao Paulo-
Descrição: dc.descriptionDepartment of General and Applied Biology Institute of Biosciences State University Julio de Mesquita Filho (UNESP)-
Descrição: dc.descriptionDepartment of General and Applied Biology Institute of Biosciences State University Julio de Mesquita Filho (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationPreparative Biochemistry and Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCold-adapted yeast-
Palavras-chave: dc.subjectenzyme production-
Palavras-chave: dc.subjectglutaminase-free asparaginase-
Palavras-chave: dc.subjectL-asparaginase-
Palavras-chave: dc.subjectLeucosporidium muscorum-
Título: dc.titleGlutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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