Synthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activity

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorLucena, Guilherme N. [UNESP]-
Autor(es): dc.creatorSantos, Caio C. dos [UNESP]-
Autor(es): dc.creatorPinto, Gabriel C. [UNESP]-
Autor(es): dc.creatorPiazza, Rodolfo D. [UNESP]-
Autor(es): dc.creatorGuedes, Wesley N. [UNESP]-
Autor(es): dc.creatorJafelicci Júnior, Miguel [UNESP]-
Autor(es): dc.creatorde Paula, Ariela V. [UNESP]-
Autor(es): dc.creatorMarques, Rodrigo Fernando C. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:35:20Z-
Data de disponibilização: dc.date.available2022-02-22T00:35:20Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-14-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jmmm.2020.167326-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/202066-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/202066-
Descrição: dc.descriptionA magnetic cross-linked enzyme aggregate (MCLEA) of cellulases and a non-magnetic analogue (CLEA) were synthesized and further investigated. The obtained MCLEA and CLEA showed a high enzyme loading efficiency of 90% and 88%, respectively. However, the MCLEA presented an activity 33% higher than the CLEA one, suggesting a catalytic enhancement effect as a result of magnetic nanoparticle presence in cross-linked cellulase aggregates structure. In addition, MCLEA shows an unusual behavior with highest enzymatic activity at low temperatures near to 37 °C. Lastly, a full factorial design (2x2) with two variables at two levels, frequency (203–420 kHz) and amplitude (3–6 kA m−1), was used in order to better investigate the effects of an alternating magnetic field (AMF) on MCLEA activity. AMF application decreased MCLEA activity, particularly in the highest values of frequency and amplitude, when compared to condition field free. In addition, frequency implied in more significant effects on MCLEA activity when compared to AMF amplitude.-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry Magnetic Materials and Colloids Laboratory-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry Institute for Bioenergy Research (IPBEN)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry Center for Monitoring and Research of the Quality of Fuels Biofuels Crude Oil and Derivatives (CEMPEQC)-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry Magnetic Materials and Colloids Laboratory-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry Institute for Bioenergy Research (IPBEN)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry Center for Monitoring and Research of the Quality of Fuels Biofuels Crude Oil and Derivatives (CEMPEQC)-
Idioma: dc.languageen-
Relação: dc.relationJournal of Magnetism and Magnetic Materials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAlternating magnetic field-
Palavras-chave: dc.subjectEnzyme immobilization-
Palavras-chave: dc.subjectMagnetic nanoparticles-
Palavras-chave: dc.subjectMagnetic supports-
Título: dc.titleSynthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activity-
Tipo de arquivo: dc.typelivro digital-
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