Synthesis of multifunctional chlorhexidine-doped thin films for titanium-based implant materials

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorIndiana University School of Dentistry-
Autor(es): dc.contributorSchool of Dentistry-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorMatos, Adaias Oliveira-
Autor(es): dc.creatorde Almeida, Amanda Bandeira-
Autor(es): dc.creatorBeline, Thamara-
Autor(es): dc.creatorTonon, Caroline C.-
Autor(es): dc.creatorCasarin, Renato Corrêa Viana-
Autor(es): dc.creatorWindsor, Lester Jack-
Autor(es): dc.creatorDuarte, Simone-
Autor(es): dc.creatorNociti, Francisco Humberto-
Autor(es): dc.creatorRangel, Elidiane Cipriano [UNESP]-
Autor(es): dc.creatorGregory, Richard L.-
Autor(es): dc.creatorBarão, Valentim Adelino Ricardo-
Data de aceite: dc.date.accessioned2022-02-22T00:35:04Z-
Data de disponibilização: dc.date.available2022-02-22T00:35:04Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.msec.2020.111289-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201985-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201985-
Descrição: dc.descriptionOur goal was to create bio-functional chlorhexidine (CHX)-doped thin films on commercially pure titanium (cpTi) discs using the glow discharge plasma approach. Different plasma deposition times (50, 35 and 20 min) were used to create bio-functional surfaces based on silicon films with CHX that were compared to the control groups [no CHX and bulk cpTi surface (machined)]. Physico-chemical and biological characterizations included: 1. Morphology, roughness, elemental chemical composition, film thickness, contact angle and surface free energy; 2. CHX-release rate; 3. Antibacterial effect on Streptococcus sanguinis biofilms at 24, 48 and 72 h; 4. Cytotoxicity and metabolic activity using fibroblasts cell culture (NIH-F3T3 cells) at 1, 2, 3 and 4 days; 5. Protein expression by NIH-F3T3 cells at 1, 2, 3 and 4 days; and 6. Co-culture assay of fibroblasts cells and S. sanguinis to assess live and dead cells on the confocal laser scanning microscopy, mitochondrial activity (XTT), membrane leakage (LDH release), and metabolic activity (WST-1 assay) at 1, 2 and 3 days of co-incubation. Data analysis showed that silicon films, with or without CHX coated cpTi discs, increased surface wettability and free energy (p < 0.05) without affecting surface roughness. CHX release was maintained over a 22-day period and resulted in a significant inhibition of biofilm growth (p < 0.05) at 48 and 72 h of biofilm formation for 50 min and 20 min of plasma deposition time groups, respectively. In general, CHX treatment did not significantly affect NIH-F3T3 cell viability (p > 0.05), whereas cell metabolism (MTT assay) was affected by CHX, with the 35 min of plasma deposition time group displaying the lowest values as compared to bulk cpTi (p < 0.05). Moreover, data analysis showed that films, with or without CHX, significantly affected the expression profile of inflammatory cytokines, including IL-4, IL-6, IL-17, IFN-y and TNF-α by NIH-F3T3 cells (p < 0.05). Co-culture demonstrated that CHX-doped film did not affect the metabolic activity, cytotoxicity and viability of fibroblasts cells (p > 0.05). Altogether, the findings of the current study support the conclusion that silicon films added with CHX can be successfully created on titanium discs and have the potential to affect bacterial growth and inflammatory markers without affecting cell viability/proliferation rates.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Prosthodontics and Periodontology Piracicaba Dental School University of Campinas (UNICAMP)-
Descrição: dc.descriptionDepartment of Biomedical Sciences and Comprehensive Care Indiana University School of Dentistry-
Descrição: dc.descriptionDepartment of Cariology Operative Dentistry and Dental Public Health Indiana University Purdue University Indianapolis School of Dentistry-
Descrição: dc.descriptionLaboratory of Technological Plasmas (LaPTec) São Paulo State University (UNESP) Science and Technology Institute of Sorocaba (ICTS)-
Descrição: dc.descriptionLaboratory of Technological Plasmas (LaPTec) São Paulo State University (UNESP) Science and Technology Institute of Sorocaba (ICTS)-
Descrição: dc.descriptionCAPES: 001-
Idioma: dc.languageen-
Relação: dc.relationMaterials Science and Engineering C-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntibacterial-
Palavras-chave: dc.subjectBiocompatible-
Palavras-chave: dc.subjectBiofilm-
Palavras-chave: dc.subjectChlorhexidine-
Palavras-chave: dc.subjectTitanium-
Título: dc.titleSynthesis of multifunctional chlorhexidine-doped thin films for titanium-based implant materials-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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