Effect of Conditioning on the Production of Inulinase by Kluyveromyces marxianus var. bulgaricus through Fed-Batch Fermentation

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorCazetta, Marcia Luciana-
Autor(es): dc.creatorMonti, Rubens-
Autor(es): dc.creatorContiero, Jonas-
Data de aceite: dc.date.accessioned2021-03-10T17:16:07Z-
Data de disponibilização: dc.date.available2021-03-10T17:16:07Z-
Data de envio: dc.date.issued2013-09-30-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2013-09-30-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2008-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.2202/1556-3758.1157-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/20194-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/20194-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionThe effects of sucrose concentration, pH, temperature and aeration rate on the production of biomass and inulinase (2,1-beta-D-fructan fructanohydrolase, EC 3.2.1.7) were investigated using fed-batch fermentation by Kluyveromyces marxianus var. bulgaricus. The best sucrose concentration for enzyme activity was 10 g L-1, whereas activity was inhibited when the sucrose concentration was 20 g L-1. Enzyme activity was strongly influenced by pH of the culture. pH 5.0 was the most suitable for both enzyme activity and cell growth, achieving 98.04 U mL-1 and 14.05 g L-1, respectively, after 72 hours of cultivation. The highest production of inulinase and biomass was observed at temperatures of 25 degrees C and 30 degrees C, whereas a decrease in activity was observed at 35 degrees C and 40 degrees C. The best aeration rate was 1.0 vvm. The increase in aeration rate or oxygen injection provided no increase in enzyme activity and biomass.-
Formato: dc.format20-
Idioma: dc.languageen-
Publicador: dc.publisherBerkeley Electronic Press-
Relação: dc.relationInternational Journal of Food Engineering-
Relação: dc.relation0,323-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectKluyveromyces marxianus var. bulgaricus-
Palavras-chave: dc.subjectsucrose-
Palavras-chave: dc.subjectinulinase-
Palavras-chave: dc.subjectcellular growth-
Palavras-chave: dc.subjectfed-batch-
Título: dc.titleEffect of Conditioning on the Production of Inulinase by Kluyveromyces marxianus var. bulgaricus through Fed-Batch Fermentation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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