Purification of Candida guilliermondii and Pichia ohmeri killer toxin as an active agent against Penicillium expansum

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorCoelho, Alexandre Rodrigo-
Autor(es): dc.creatorTachi, Masahico-
Autor(es): dc.creatorPagnocca, Fernando Carlos-
Autor(es): dc.creatorAndrade Nobrega, Gisele Maria-
Autor(es): dc.creatorHoffmann, Fernando Leite-
Autor(es): dc.creatorHarada, Ken-ichi-
Autor(es): dc.creatorHirooka, Elisa Yoko-
Data de aceite: dc.date.accessioned2021-03-10T17:16:05Z-
Data de disponibilização: dc.date.available2021-03-10T17:16:05Z-
Data de envio: dc.date.issued2013-09-30-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2013-09-30-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2009-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/02652030802227227-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/20181-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/20181-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUGF-SETI-
Descrição: dc.descriptionJICA (Japan International Cooperation Agency)-
Descrição: dc.descriptionAn antifungal assay with cell-free culture supernatant of Pichia ohmeri 158 and Candida guilliermondii P3 was tested against Penicillium expansum strain #2 at 25C by measuring hyphal length and percentage conidia germination. C. guilliermondii was more effective against P. expansum conidia germination (58.15% inhibition), while P. ohmeri showed higher inhibition of mycelial growth (66.17%), indicating a probable mechanism associated with killer activity. This killer toxin (molecular mass 3 kDa) was partially purified by normal phase HPLC, using TSKgel Amide-80 analytical and preparative columns. Compared with crude extract, the killer toxin eluted from the post analytical column significantly inhibited P. expansum:% inhibition rose from 42.16 to 90.93% (C. guilliermondii) and 39.32 to 91.12% (P. ohmeri) (p 0.05). The one-step purification process was adequate in isolating killer toxin from culture supernatant and also increased anti-Penicillium activity.-
Formato: dc.format73-81-
Idioma: dc.languageen-
Publicador: dc.publisherTaylor & Francis Ltd-
Relação: dc.relationFood Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectPenicillium expansum-
Palavras-chave: dc.subjectantagonist yeasts-
Palavras-chave: dc.subjectkiller toxin-
Palavras-chave: dc.subjectBiological control-
Título: dc.titlePurification of Candida guilliermondii and Pichia ohmeri killer toxin as an active agent against Penicillium expansum-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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